The Number of Frames on ECG-Gated ¹⁸F-FDG Small Animal PET Has a Significant Impact on LV Systolic and Diastolic Functional Parameters.

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Molecular Imaging Pub Date : 2021-12-06 eCollection Date: 2021-01-01 DOI:10.1155/2021/4629459

Christoph Eissler, Rudolf A Werner, Paula Arias-Loza, Naoko Nose, Xinyu Chen, Martin G Pomper, Steven P Rowe, Constantin Lapa, Andreas K Buck, Takahiro Higuchi

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引用次数: 2

Abstract

Objectives: This study is aimed at investigating the impact of frame numbers in preclinical electrocardiogram- (ECG-) gated ¹⁸F-fluorodeoxyglucose (¹⁸F-FDG) positron emission tomography (PET) on systolic and diastolic left ventricular (LV) parameters in rats.

Methods: ¹⁸F-FDG PET imaging using a dedicated small animal PET system with list mode data acquisition and continuous ECG recording was performed in diabetic and control rats. The list-mode data was sorted and reconstructed with different numbers of frames (4, 8, 12, and 16) per cardiac cycle into tomographic images. Using an automatic ventricular edge detection software, left ventricular (LV) functional parameters, including ejection fraction (EF), end-diastolic (EDV), and end-systolic volume (ESV), were calculated. Diastolic variables (time to peak filling (TPF), first third mean filling rate (1/3 FR), and peak filling rate (PFR)) were also assessed.

Results: Significant differences in multiple parameters were observed among the reconstructions with different frames per cardiac cycle. EDV significantly increased by numbers of frames (353.8 ± 57.7 μl^∗, 380.8 ± 57.2 μl^∗, 398.0 ± 63.1 μl^∗, and 444.8 ± 75.3 μl at 4, 8, 12, and 16 frames, respectively; ^∗ P < 0.0001 vs. 16 frames), while systolic (EF) and diastolic (TPF, 1/3 FR and PFR) parameters were not significantly different between 12 and 16 frames. In addition, significant differences between diabetic and control animals in 1/3 FR and PFR in 16 frames per cardiac cycle were observed (P < 0.005), but not for 4, 8, and 12 frames.

Conclusions: Using ECG-gated PET in rats, measurements of cardiac function are significantly affected by the frames per cardiac cycle. Therefore, if you are going to compare those functional parameters, a consistent number of frames should be used.

Abstract Image

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心电图门控18F-FDG小动物PET帧数对左室收缩和舒张功能参数有显著影响。

目的:探讨临床前心电图(ECG)门控18f -氟脱氧葡萄糖(18F-FDG)正电子发射断层扫描(PET)帧数对大鼠左室(LV)收缩和舒张参数的影响。方法:采用专用小动物PET系统对糖尿病大鼠和对照组大鼠进行18F-FDG PET成像，采用列表模式采集数据并连续记录心电图。对每个心动周期的不同帧数(4、8、12、16帧)的列表模式数据进行排序并重构为层析图像。使用心室边缘自动检测软件，计算左心室(LV)功能参数，包括射血分数(EF)、舒张末期(EDV)和收缩末期容积(ESV)。还评估了舒张期变量(至充盈高峰时间(TPF)、前三次平均充盈率(1/3 FR)和充盈高峰率(PFR))。结果:每心动周期不同帧数重建的多个参数有显著性差异。在4、8、12和16帧时，EDV的帧数分别为353.8±57.7 μl∗、380.8±57.2 μl∗、398.0±63.1 μl∗和444.8±75.3 μl;(∗P < 0.0001 vs. 16帧)，而收缩期(EF)和舒张期(TPF, 1/3 FR和PFR)参数在12和16帧之间无显著差异。此外，糖尿病动物和对照动物在每心动周期16帧的1/3 FR和PFR中观察到显著差异(P < 0.005)，但在4、8和12帧时则无显著差异。结论:在大鼠中使用心电图门控PET，心功能的测量受到每个心动周期帧数的显著影响。因此，如果要比较这些功能参数，应该使用一致数量的帧。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology

自引率

3.60%

发文量

期刊介绍： Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.