Calcineurin Aα Contributes to IgE-Dependent Mast-Cell Mediator Secretion in Allergic Inflammation.

IF 4.7 3区 医学 Q2 IMMUNOLOGY
Journal of Innate Immunity Pub Date : 2022-01-01 Epub Date: 2021-11-26 DOI:10.1159/000520040
Edwin Leong, Zheng Pang, Andrew W Stadnyk, Tong-Jun Lin
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引用次数: 2

Abstract

Mast cells (MCs) are key mediators of allergic inflammation through the activation of cross-linked immunoglobulin E (IgE) bound to the high-affinity IgE receptor (FcϵRI) on the cell surface, leading to the release of biologically potent mediators, either from preformed granules or newly synthesized. Pharmacological inhibitors have been developed to target a key signaling protein phosphatase in this pathway, calcineurin, yet there is a lack of genetic and definitive evidence for the various isoforms of calcineurin subunits in FcϵRI-mediated responses. In this study, we hypothesized that deficiency in the calcineurin Aα isoform will result in a decreased allergic immune response by the MCs. In a model of passive cutaneous anaphylaxis, there was a reduction in vascular permeability in MC-deficient mouse tissues reconstituted with calcineurin subunit A (CnAα) gene-knockout (CnAα-/-) MCs, and in vitro experiments identified a significant reduction in release of preformed mediators from granules. Furthermore, released levels of de novo synthesized cytokines were reduced upon FcϵRI activation of CnAα-/- MCs in vitro. Characterizing the mechanisms associated with this deficit response, we found a significant impairment of nuclear factor of kappa light polypeptide gene enhancer in B cell phosphorylation and impaired nuclear factor kappa-light-chain-enhancer of activated B-cell inhibitor alpha (NF-κB) activation. Thus, we concluded that CnAα contributes to the release of preformed mediators and newly synthesized mediators from FcϵRI-mediated activation of MCs, and this regulation includes NF-κB signaling.

钙调磷酸酶Aα参与过敏性炎症中ige依赖性肥大细胞介质的分泌。
肥大细胞(MCs)是过敏性炎症的关键介质,通过激活与细胞表面高亲和力IgE受体(FcϵRI)结合的交联免疫球蛋白E (IgE),导致从预先形成的颗粒或新合成的生物强效介质的释放。目前已经开发出针对该途径中关键信号蛋白磷酸酶钙调神经磷酸酶的药物抑制剂,但缺乏钙调神经磷酸酶亚基在FcϵRI-mediated反应中的各种亚型的遗传和明确证据。在这项研究中,我们假设钙调神经磷酸酶a α异构体的缺乏会导致MCs的过敏免疫反应降低。在被动皮肤过敏反应模型中,用钙调神经磷酸酶亚基a (CnAα)基因敲除(CnAα-/-) MCs重建的mc缺陷小鼠组织中,血管通透性降低,体外实验发现颗粒中预形成介质的释放显著减少。此外,在体外FcϵRI激活na - α-/- MCs后,新生合成的细胞因子释放水平降低。为了表征这种缺陷反应的相关机制,我们发现kappa轻多肽基因增强子的核因子在B细胞磷酸化中的显著损伤,以及活化的B细胞抑制剂α (NF-κB)活化的核因子kappa轻链增强子的显著损伤。因此,我们得出结论,在FcϵRI-mediated活化MCs的过程中,na - α参与了预形成介质和新合成介质的释放,这种调节包括NF-κB信号传导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Innate Immunity
Journal of Innate Immunity 医学-免疫学
CiteScore
10.50
自引率
1.90%
发文量
35
审稿时长
7.5 months
期刊介绍: The ''Journal of Innate Immunity'' is a bimonthly journal covering all aspects within the area of innate immunity, including evolution of the immune system, molecular biology of cells involved in innate immunity, pattern recognition and signals of ‘danger’, microbial corruption, host response and inflammation, mucosal immunity, complement and coagulation, sepsis and septic shock, molecular genomics, and development of immunotherapies. The journal publishes original research articles, short communications, reviews, commentaries and letters to the editors. In addition to regular papers, some issues feature a special section with a thematic focus.
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