Identification of proAKAP4 concentration variations in dromedary sperm and their correlation with monthly semen parameters.

Reproduction & Fertility Pub Date : 2021-09-23 eCollection Date: 2021-12-01 DOI:10.1530/RAF-21-0055
Clara Malo, Sara Carracedo, Maryse Delehedde, Nicolas Sergeant, Julian Alexandra Skidmore
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引用次数: 4

Abstract

ProAKAP4 is synthetized as a precursor polypeptide that must be converted into mature AKAP4 in living spermatozoa and is considered as a functional marker of spermatozoa. The gene is well-conserved in mammals although uncharacterized in Camelidae. In the present study, we investigate the expression metabolism of proAKAP4 and AKAP4 proteins and evaluate their seasonal dynamics relative to semen quality in dromedary camels. Semen parameters including volume and viscosity and characteristics of sperm including concentration, total production, total and progressive motility, vitality, acrosome integrity and morphological abnormalities were assessed in semen samples collected weekly from six camels during the rutting season, from November to April. Only total sperm production varied, peaking in January. Both the precursor proAKAP4 and AKAP4 proteins were investigated and shown to express biochemical properties similar to those described in other mammals. ProAKAP4 concentrations expressed in ng/10 million spermatozoa as assayed using a specific ELISA showed a strong positive correlation with ejaculate volume (P = 0.045), viscosity (P < 0.001) and sperm total motility (P = 0.049). Furthermore, their concentrations exhibited clear seasonal variations in camel semen. In conclusion, the assessment of proAKAP4 concentrations in camel sperm provides a novel parameter to assess sperm quality. Further studies should be performed to investigate proAKAP4 concentrations relative to fertility in Camelidae that may help to define the right time for mating and semen collection and increase the success of breeding programs.

Lay summary: Breeding related to the seasons/time of year in the camel has been reported in several studies. A better knowledge of semen quality during the breeding season would assist in determining the best period for mating in camels. However, conventional sperm parameters are held to be unsatisfactory because they cannot predict breeding potential. ProAKAP4 a sperm-specific protein has been described as a functional marker of sperm and a key fertility marker in several species but has not been described in camels. Motility or membrane integrity parameters of semen collected throughout the breeding season and also the presence of proAKAP4 protein were investigated. ProAKAP4 was identified for the first time in camels and their concentrations exhibited clear seasonal variations in camel semen showing strong correlations with ejaculate volume and total motility and viscosity. Further studies should be performed to investigate proAKAP4 concentrations relative to fertility in camels to define the right time for mating and increase the success of breeding programs.

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单峰精子中proAKAP4浓度变化及其与月度精液参数的相关性研究。
ProAKAP4是一种前体多肽,必须在活精子中转化为成熟的AKAP4,被认为是精子的功能标记物。该基因在哺乳动物中保存良好,但在骆驼科中未发现。在本研究中,我们研究了proAKAP4和AKAP4蛋白的表达代谢,并评估了它们与单峰骆驼精液质量的季节性动态关系。在11月至4月的发情期,每周采集6只骆驼的精液样本,评估精液的体积和粘度等参数,以及精子的浓度、总量、总运动性和进展运动性、活力、顶体完整性和形态异常等特征。只有精子总量有所变化,在一月份达到峰值。我们研究了前体proAKAP4和AKAP4蛋白,发现它们表达的生化特性与其他哺乳动物相似。以ng/ 1000万精子为单位表达的ProAKAP4浓度与射精量(P = 0.045)、黏度(P < 0.001)和精子总活动力(P = 0.049)呈显著正相关。此外,它们的浓度在骆驼精液中表现出明显的季节性变化。总之,评估骆驼精子中proAKAP4的浓度为评估精子质量提供了一个新的参数。进一步研究proAKAP4浓度与Camelidae繁殖能力的关系可能有助于确定交配和精液采集的正确时间,并提高育种计划的成功率。几项研究报告了骆驼的繁殖与季节/一年中的时间有关。更好地了解繁殖季节的精液质量将有助于确定骆驼的最佳交配期。然而,传统的精子参数被认为是不令人满意的,因为它们不能预测繁殖潜力。ProAKAP4是一种精子特异性蛋白,在一些物种中被描述为精子的功能标记和关键的生育标记,但在骆驼中尚未被描述。研究了整个繁殖季节采集的精液的运动性或膜完整性参数以及proAKAP4蛋白的存在。ProAKAP4首次在骆驼中被鉴定出来,其浓度在骆驼精液中表现出明显的季节性变化,与射精量、总运动性和粘度有很强的相关性。应进一步研究proAKAP4浓度与骆驼生育能力的关系,以确定合适的交配时间,提高育种计划的成功率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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