A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath.

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Ville A Palomäki, Vesa Koivukangas, Sanna Meriläinen, Petri Lehenkari, Tuomo J Karttunen
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引用次数: 8

Abstract

Changes in adipose tissue morphology, depicted by cell morphology alterations such as enlargement of fat cells, always accompany over-weight and obesity. The variables related to cell size have been shown to associate with low-grade inflammation of adipose tissue and common obesity-related comorbidities including metabolic syndrome and type 2 diabetes. Quantifying fat cell morphology from images of histological specimens can be tedious. Here, we present a straightforward method for the task using the free open-source software QuPath with its inbuilt tools only. Measurements of human adipose tissue samples with the described protocol showed an excellent correlation with those obtained with ImageJ software with Adipocyte Tools plugin combined with manual correction of misdetections. Intraclass correlation between the two methods was at good to excellent level. The method described here can be applied to considerably large tissue areas, even whole-slide analysis.

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Abstract Image

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使用开源软件QuPath进行脂肪细胞大小和计数分析的简单方法。
脂肪组织形态的改变,由细胞形态的改变所描述,如脂肪细胞的增大,总是伴随着超重和肥胖。与细胞大小相关的变量已被证明与脂肪组织的低度炎症和常见的肥胖相关合并症(包括代谢综合征和2型糖尿病)有关。从组织学标本的图像定量脂肪细胞形态可能是繁琐的。在这里,我们提出了一种简单的方法,使用免费的开源软件QuPath及其内置工具。使用所描述的方案测量的人体脂肪组织样品与使用ImageJ软件与adicyte Tools插件结合人工校正误检获得的结果具有良好的相关性。两种方法的类内相关性处于良好至优秀水平。这里描述的方法可以应用于相当大的组织区域,甚至整个幻灯片分析。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
期刊介绍: ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/​Abstracted: Web of Science SCIE Scopus CAS INSPEC Portico
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