Alfalfa saponins inhibit oxidative stress-induced cell apoptosis through the MAPK signaling pathway.

IF 5.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Redox Report Pub Date : 2022-12-01 DOI:10.1080/13510002.2021.2017681

Yalei Cui, Fen Li, Xiaoyan Zhu, Junying Xu, Abaidullah Muhammad, Yanyan Chen, Defeng Li, Boshuai Liu, Chengzhang Wang, Zhichang Wang, Sen Ma, Xule Liu, Yinghua Shi

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引用次数: 0

Abstract

Background: Oxidative stress could seriously affect the growth performance of piglets. As natural extracts of Alfalfa (Medicago sativa), alfalfa saponins have been shown to function as antioxidants in piglets in vivo. However, few studies have investigated the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells in vitro. In the current study, piglets' small intestinal epithelial cell line (IPEC-J2) was explored to investigate the protective effects of alfalfa saponins on injured cells induced by H₂O₂.

Methods: To investigate the effects and mechanism of alfalfa saponins against oxidative stress in piglet cells, the cell viability, activity of antioxidant enzymes, LDH and the amount of MDA were detected in H₂O₂-treated cells after the cells were pre-incubated with alfalfa saponins. The mechanism of alfalfa saponins against H₂O₂-induced oxidative cell damage was explored by detecting the expression of mitochondrial apoptosis-related proteins. Furthermore, the signaling pathway of alfalfa saponins in IPEC-J2 cells under oxidative stress was also investigated.

Results: The results indicated that alfalfa saponins could rescue cell viability, elevate the activity of antioxidant enzymes and down-regulate the activity of LDH and the amount of MDA in H₂O₂-induced cells.

Conclusion: Alfalfa saponins could inhibit oxidative stress-induced cell mitochondrial apoptosis through the MAPK signaling pathway, thereby providing a new method for improving antioxidant stress ability by means of nutritional regulation.

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紫花苜蓿皂苷通过 MAPK 信号通路抑制氧化应激诱导的细胞凋亡。

背景：氧化应激会严重影响仔猪的生长性能：氧化应激会严重影响仔猪的生长性能。作为紫花苜蓿（Medicago sativa）的天然提取物，苜蓿皂苷已被证明在仔猪体内具有抗氧化功能。然而，很少有研究调查了苜蓿皂苷在体外对仔猪细胞氧化应激的影响和机制。本研究以仔猪小肠上皮细胞系（IPEC-J2）为研究对象，探讨苜蓿皂苷对 H2O2 诱导的损伤细胞的保护作用：为了研究紫花苜蓿皂苷对仔猪细胞氧化应激的作用及其机制，用紫花苜蓿皂苷预孵育H2O2处理的细胞，检测细胞活力、抗氧化酶活性、LDH和MDA的含量。通过检测线粒体凋亡相关蛋白的表达，探讨了苜蓿皂苷抗 H2O2 诱导的细胞氧化损伤的机制。此外，还研究了紫花苜蓿皂苷在氧化应激下 IPEC-J2 细胞中的信号传导途径：结果表明，紫花苜蓿皂苷可以挽救细胞活力，提高抗氧化酶的活性，下调 H2O2 诱导细胞中 LDH 的活性和 MDA 的含量：紫花苜蓿皂甙可通过MAPK信号通路抑制氧化应激诱导的细胞线粒体凋亡，从而为通过营养调控提高细胞抗氧化能力提供了一种新方法。

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来源期刊

Redox Report 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.