Effect of the natural flavonoids myricetin and dihydromyricetin on the wound healing process in vitro.

IF 2 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Journal of applied biomedicine Pub Date : 2021-09-01 Epub Date: 2021-08-10 DOI:10.32725/jab.2021.017

Renata Sklenarova, Marika Svrckova, Petr Hodek, Jitka Ulrichova, Jana Frankova

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引用次数: 10

Abstract

Myricetin (MYR) and dihydromyricetin (DHM) are classified as natural flavonoids. Both substances are known for their anti-inflammatory and antioxidant properties. In this study, an in vitro model of inflammation was demonstrated on monolayers of scratched fibroblasts or keratinocytes exposed to LPS from Pseudomonas aeruginosa for six hours. MYR and DHM were subsequently applied to the cells for 24 hours at sub toxic concentrations (5-15 µM). Inflammatory parameters were analysed in collected cell medium and lysate after the incubation period using the Enzyme-Linked ImmuneSorbent Assay (ELISA) and Western blot. Both flavonoids inhibit the production of pro-inflammatory cytokines (IL-6, IL-8) in LPS-stimulated skin cells as well as the decreased level of MMP-1 in fibroblasts. However, the application of MYR and DHM dose dependently increased the level of MMP-1 in keratinocytes. In our experiments, we focused on the anti-glycation activity of MYR and DHM, where the higher concentration of MYR seems to be more effective.

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天然黄酮类杨梅素和二氢杨梅素对体外创面愈合的影响。

杨梅素(MYR)和二氢杨梅素(DHM)属于天然类黄酮。这两种物质都以其抗炎和抗氧化特性而闻名。在这项研究中，在铜绿假单胞菌LPS下暴露6小时的划痕成纤维细胞或角质形成细胞单层上建立了体外炎症模型。随后将MYR和DHM以亚毒性浓度(5-15µM)作用于细胞24小时。用酶联免疫吸附试验(ELISA)和Western blot分析收集的细胞培养基和孵育后的裂解液中的炎症参数。两种黄酮类化合物均抑制lps刺激皮肤细胞中促炎细胞因子(IL-6、IL-8)的产生，并降低成纤维细胞中MMP-1的水平。然而，MYR和DHM剂量依赖性地增加了角质形成细胞中MMP-1的水平。在我们的实验中，我们重点研究了MYR和DHM的抗糖基化活性，其中MYR浓度越高似乎越有效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of applied biomedicine PHARMACOLOGY & PHARMACY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.