A biochemical approach to the anti-inflammatory, antioxidant and antiapoptotic potential of beta-carotene as a protective agent against bromobenzene-induced hepatotoxicity in female Wistar albino rats.

IF 2 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Journal of applied biomedicine Pub Date : 2020-08-01 Epub Date: 2020-08-10 DOI:10.32725/jab.2020.011
Priya Josson Akkara, Evan Prince Sabina
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引用次数: 4

Abstract

Bromobenzene is a compound which has contributed much in understanding the mechanisms involved in xenobiotic hepatotoxicity induced by drugs and environment pollutants. In the present study, the protective and ameliorative effect of beta-carotene was investigated against bromobenzene-induced hepatotoxicity and compared with silymarin, a standard hepatoprotective reference drug. Beta-carotene (10 mg/kg b.w. p.o.) was administered to the rats for 9 days before intragastric intubation of bromobenzene (10 mmol/kg b.w.). Liver marker enzymes (aspartate transaminase, alanine transaminase and alkaline phosphatase), total protein content, bilirubin, total cholesterol, high-density lipoproteins, triglycerides, antioxidant status (reduced glutathione, superoxide dismutase, catalase, glutathione-S-transferase and glutathione peroxidase) were assessed along with histopathological analysis. ELISA was performed for analysing the levels of cytokines such as TNF-α, IL-1β and IL-6 in serum and in the liver. Caspase-3, COX-2 and NF-κB were evaluated by Western blotting. Administration of bromobenzene resulted in elevated levels of liver marker enzymes, bilirubin, lipid peroxidation and cytokines but deterioration in total protein content, antioxidant levels and histopathological conditions. Pre-treatment with beta-carotene not only significantly decreased the levels of liver markers, lipid peroxidation and cytokines but also improved histo-architecture and increased antioxidant levels minimising oxidative stress, and reduced factors contributing to apoptosis. This significant reversal of the biochemical changes on pre-treatment with beta-carotene in comparison with rats administered with bromobenzene clearly demonstrates that beta-carotene possesses promising hepatoprotective effect through its antioxidant, anti-inflammatory and antiapoptotic activity and hence is suggested as a potential therapeutic agent for protection from bromobenzene.

生物化学方法研究β -胡萝卜素对溴苯诱导的雌性Wistar白化大鼠肝毒性的抗炎、抗氧化和抗凋亡作用。
溴苯是一种化合物,在了解药物和环境污染物引起的外源性肝毒性机制方面做出了重要贡献。本研究考察了β -胡萝卜素对溴苯所致肝毒性的保护和改善作用,并与标准保肝参比药物水飞蓟素进行了比较。大鼠灌胃10 mmol/kg b.w.的溴苯前,连续9天给予β -胡萝卜素(10 mg/kg b.w.)。评估肝脏标志物酶(天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶)、总蛋白含量、胆红素、总胆固醇、高密度脂蛋白、甘油三酯、抗氧化状态(还原性谷胱甘肽、超氧化物歧化酶、过氧化氢酶、谷胱甘肽- s转移酶和谷胱甘肽过氧化物酶)并进行组织病理学分析。ELISA法分析血清和肝脏中TNF-α、IL-1β和IL-6等细胞因子水平。Western blotting检测Caspase-3、COX-2、NF-κB。溴苯导致肝脏标记酶、胆红素、脂质过氧化和细胞因子水平升高,但总蛋白含量、抗氧化水平和组织病理状况恶化。β -胡萝卜素预处理不仅能显著降低肝脏标志物、脂质过氧化和细胞因子的水平,还能改善组织结构,增加抗氧化水平,最大限度地减少氧化应激,并减少导致细胞凋亡的因素。与溴苯处理大鼠相比,β -胡萝卜素处理前的生化变化明显逆转,这清楚地表明β -胡萝卜素通过其抗氧化、抗炎和抗凋亡活性具有良好的肝保护作用,因此被认为是一种潜在的溴苯保护治疗剂。
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来源期刊
Journal of applied biomedicine
Journal of applied biomedicine PHARMACOLOGY & PHARMACY-
CiteScore
2.40
自引率
7.70%
发文量
13
审稿时长
>12 weeks
期刊介绍: Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.
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