Studying cell volume beyond cell volume.

4区 生物学 Q4 Biochemistry, Genetics and Molecular Biology
Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-10-02 DOI:10.1016/bs.ctm.2021.08.001
Michael A Model
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引用次数: 0

Abstract

The first part of the paper describes two simple microscopic techniques that we use in our laboratory. One measures cell volumes in adherent cultures and the other measures cell dry mass; both measurements are done on the same instrument (a standard bright-field transmission microscope with only one or two narrow-band color filters added) and on the same cells. The reason for combining cell volume with dry mass is that the ratio of the two-dry mass concentration (MC)-is an important and insufficiently utilized biological parameter. We then describe a few applications of MC. The available experimental data strongly suggest its critical role in biological processes, including cell volume regulation. For example, most eukaryotic cells have surprisingly similar values of MC. Moreover, MC (and not cell volume) is tightly controlled in growing cell cultures at highly variable external osmolarities. We review the results showing that elevation of MC is a direct cause of shrinkage-induced apoptosis. Also, by focusing on MC, one can study heterogenous processes, such as necrotic swelling, or discriminate between apoptotic dehydration and the loss of cell fragments.

研究细胞体积以外的细胞体积。
本文的第一部分描述了我们在实验室中使用的两种简单的显微技术。一种测量贴壁培养中的细胞体积,另一种测量细胞干质量;这两种测量都是在相同的仪器(一个标准的亮场透射显微镜,只加了一个或两个窄带滤色片)和相同的细胞上完成的。将细胞体积与干质量结合起来的原因是两者的比值——干质量浓度(MC)——是一个重要但未得到充分利用的生物学参数。然后我们描述了MC的一些应用。现有的实验数据有力地表明它在生物过程中起着关键作用,包括细胞体积调节。例如,大多数真核细胞具有惊人的相似的MC值。此外,在高度可变的外部渗透压下生长的细胞培养中,MC(而不是细胞体积)受到严格控制。我们回顾了研究结果,表明MC升高是收缩性细胞凋亡的直接原因。此外,通过关注MC,人们可以研究异质过程,如坏死性肿胀,或区分凋亡性脱水和细胞片段丢失。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current topics in membranes
Current topics in membranes 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
10
审稿时长
>12 weeks
期刊介绍: Current Topics in Membranes provides a systematic, comprehensive, and rigorous approach to specific topics relevant to the study of cellular membranes. Each volume is a guest edited compendium of membrane biology.
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