Expression of Chicken NK-Lysin and Its Role in Chicken Coccidiosis Induced by Eimeria necatrix.

IF 1.4 4区医学 Q3 PARASITOLOGY

Korean Journal of Parasitology Pub Date : 2021-10-01 Epub Date: 2021-10-22 DOI:10.3347/kjp.2021.59.5.439

Woo Hyun Kim, Wongi Min, Kwang Il Park, Hyun S Lillehoj, Cherry P Fernandez-Colorado, Rochelle A Flores, Paula Leona T Cammayo, Binh Thanh Nguyen

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引用次数: 2

Abstract

Coccidiosis in chickens is an intestinal parasitic disease caused by protozoan parasites named Eimeria spp. In some Eimeria infections, intestinal lymphocytes are known to highly express chicken NK-lysin (cNK-lysin), an antimicrobial peptide with anticoccidial activity. Therefore, this study aims to investigate the expression of cNK-lysin in E. necatrix-infected chickens and its role in E. necatrix infection. The expression of cNK-lysin transcript was significantly increased in E. necatrix sporozoites-treated lymphocytes. In E. necatrix infection, cNK-lysin transcript was induced in intestinal lymphocytes but not in the spleen. The recombinant cNK-lysin exhibited anticoccidial activity against E. necatrix sporozoites as well as immunomodulatory activity on macrophages by inducing proinflammatory cytokines. These results indicated that E. necatrix infection induces high local expression of cNK-lysin and the secreted cNK-lysin helps protect coccidiosis.

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鸡nk -溶素在鸡球虫病中的表达及其作用。

鸡球虫病是由艾美耳球虫引起的一种肠道寄生虫病，在某些艾美耳球虫感染中，肠道淋巴细胞高表达鸡NK-lysin (cNK-lysin)，这是一种具有抗球虫活性的抗菌肽。因此，本研究旨在探讨cNK-lysin在necatrix感染鸡体内的表达及其在necatrix感染中的作用。cNK-lysin转录本的表达显著增加。在necatrie感染时，在肠淋巴细胞中诱导cNK-lysin转录，而在脾脏中不诱导。重组cNK-lysin具有抗球虫活性，并通过诱导促炎细胞因子对巨噬细胞具有免疫调节作用。这些结果表明，球虫感染可诱导cNK-lysin在局部高表达，其分泌的cNK-lysin对球虫病有保护作用。

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来源期刊

Korean Journal of Parasitology PARASITOLOGY-

CiteScore

2.80

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Korean Journal of Parasitology is the official journal paperless, on-line publication after Vol. 53, 2015 of The Korean Society for Parasitology and Tropical Medicine. Abbreviated title is ‘Korean J Parasitol’. It was launched in 1963. It contains original articles, case reports, brief communications, reviews or mini-reviews, book reviews, and letters to the editor on parasites of humans and animals, vectors, host-parasite relationships, zoonoses, and tropical medicine. It is published bimonthly in February, April, June, August, October, and December each year. Supplement numbers are at times published. All of the manuscripts are peer-reviewed.