A polydopamine-assisted strontium-substituted apatite coating for titanium promotes osteogenesis and angiogenesis via FAK/MAPK and PI3K/AKT signaling pathways

IF 8.1 1区工程技术 Q1 MATERIALS SCIENCE, BIOMATERIALS

Materials science & engineering. C, Materials for biological applications Pub Date : 2021-12-01 DOI:10.1016/j.msec.2021.112482

Yiting Sun , Yaxin Li , Yu Zhang , Tiange Wang , Kaili Lin , Jiaqiang Liu

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引用次数: 25

Abstract

Early osteointegration is essential for biomedical implants. Surface modifications can significantly compensate for an implant's lack of biocompatibility and osteo-differentiation. They can also be designed to promote angiogenesis in order to assist osteogenesis and ultimately facilitate bone regeneration. In this study, a polydopamine-assisted strontium-substituted apatite coating (Ti@PDA + SrHA) was fabricated on a multifunctional titanium implant to induce both angiogenic and osteogenic abilities for rapid osseointegration. Polydopamine and Sr-substituted hydroxyapatite were coated on the implant through biomineralization. The in vitro results showed that Ti@PDA + SrHA improved cell adhesion and increased the proliferation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs). Ti@PDA + SrHA upregulated the expression of ALP activity and osteogenic genes in rBMSCs and elevated angiogenic genes in both rBMSCs and HUVECs. Mechanically, the FAK/MAPK signaling pathway was activated in rBMSCs, and the PI3K/AKT signaling pathway was activated in both rBMSCs and HUVECs. Consistent with these findings, Ti@PDA + SrHA accelerated new bone formation and rapid osseointegration in the femoral condyle implantation study with good stability. Overall, we fabricated a multifunctional biocompatible implant with better angiogenic and osteogenic performance compared to the non-coated implant.

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多多巴胺辅助的锶取代磷灰石涂层通过FAK/MAPK和PI3K/AKT信号通路促进骨生成和血管生成

早期骨整合对生物医学植入物至关重要。表面修饰可以显著弥补种植体生物相容性和骨分化的不足。它们也可以被设计成促进血管生成，以帮助成骨，最终促进骨再生。在这项研究中，在多功能钛种植体上制备了一种聚多巴胺辅助锶取代磷灰石涂层(Ti@PDA + SrHA)，以诱导血管生成和成骨能力，实现快速骨整合。聚多巴胺和sr取代羟基磷灰石通过生物矿化涂覆在种植体上。体外实验结果显示，Ti@PDA + SrHA可改善大鼠骨髓间充质干细胞(rBMSCs)和人脐静脉内皮细胞(HUVECs)的细胞粘附，促进细胞增殖。Ti@PDA + SrHA上调rBMSCs中ALP活性和成骨基因的表达，上调rBMSCs和huvec中血管生成基因的表达。机械地，FAK/MAPK信号通路在rBMSCs中被激活，PI3K/AKT信号通路在rBMSCs和huvec中都被激活。与这些发现一致，Ti@PDA + SrHA在股骨髁植入研究中加速新骨形成和快速骨整合，具有良好的稳定性。总的来说，我们制造了一种多功能生物相容性种植体，与无涂层种植体相比，它具有更好的血管生成和成骨性能。

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来源期刊

Materials science & engineering. C, Materials for biological applications MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

12.60

自引率

0.00%

发文量

审稿时长

3.3 months

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