In vivo microelectrode monitoring of real-time hydrogen concentration in different tissues of rats after inhaling hydrogen gas.

IF 3 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Medical Gas Research Pub Date : 2022-07-01 DOI:10.4103/2045-9912.330694

Bo-Yan Liu, Jun-Li Xue, Qian-Qian Gu, Min Zhao, Meng-Yu Zhang, Ming-Yue Wang, Yun Wang, Shu-Cun Qin

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引用次数: 7

Abstract

Medical effects of hydrogen have been reported in many studies. Due to difficulties in measuring hydrogen concentration in vivo after intake and high explosive risks of hydrogen, studies about dose-response relationships and tissue concentrations of hydrogen are few. Here, for the first time, we monitored real-time hydrogen concentrations in different tissues in rats including brain, liver, spleen, kidney, thigh muscle, inguinal white adipose tissue, and gonadal white adipose tissue after inhaling different concentrations of hydrogen (4%, 42%, and 67%) using an electrochemical sensor. Hydrogen concentrations in the same tissue showed a dose-dependent response. The equilibrium concentration values were highest in the brain and lowest in the thigh muscle. The saturation and desaturation curves changed more slowly in the thigh muscle and white adipose tissues than in other tissues. These results provide fundamental information for the selection of hydrogen dose applications in basic research and clinical trials. The experiments were approved by the Laboratory Animal Ethics Committee of Shandong First Medical University & Shandong Academy of Medical Sciences (No. 2020-1028) on March 18, 2020.

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大鼠吸入氢气后不同组织内氢浓度实时微电极监测。

许多研究报告了氢的医学作用。由于摄入氢气后体内氢气浓度难以测量，且氢气具有较高的爆炸危险性，因此关于氢气的剂量-反应关系和组织浓度的研究很少。在这里，我们首次使用电化学传感器实时监测大鼠吸入不同浓度的氢(4%、42%和67%)后，脑、肝、脾、肾、大腿肌肉、腹股沟白色脂肪组织和性腺白色脂肪组织中的氢浓度。同一组织中的氢浓度表现出剂量依赖性反应。平衡浓度在大脑中最高，在大腿肌肉中最低。大腿肌肉和白色脂肪组织的饱和和去饱和曲线变化较其他组织慢。这些结果为基础研究和临床试验中氢剂量的选择提供了依据。本实验于2020年3月18日经山东第一医科大学、山东省医学科学院实验动物伦理委员会(No. 2020-1028)批准。

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来源期刊

Medical Gas Research MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

5.10

自引率

13.80%

发文量

期刊介绍： Medical Gas Research is an open access journal which publishes basic, translational, and clinical research focusing on the neurobiology as well as multidisciplinary aspects of medical gas research and their applications to related disorders. The journal covers all areas of medical gas research, but also has several special sections. Authors can submit directly to these sections, whose peer-review process is overseen by our distinguished Section Editors: Inert gases - Edited by Xuejun Sun and Mark Coburn, Gasotransmitters - Edited by Atsunori Nakao and John Calvert, Oxygen and diving medicine - Edited by Daniel Rossignol and Ke Jian Liu, Anesthetic gases - Edited by Richard Applegate and Zhongcong Xie, Medical gas in other fields of biology - Edited by John Zhang. Medical gas is a large family including oxygen, hydrogen, carbon monoxide, carbon dioxide, nitrogen, xenon, hydrogen sulfide, nitrous oxide, carbon disulfide, argon, helium and other noble gases. These medical gases are used in multiple fields of clinical practice and basic science research including anesthesiology, hyperbaric oxygen medicine, diving medicine, internal medicine, emergency medicine, surgery, and many basic sciences disciplines such as physiology, pharmacology, biochemistry, microbiology and neurosciences. Due to the unique nature of medical gas practice, Medical Gas Research will serve as an information platform for educational and technological advances in the field of medical gas.