Embryonic expression patterns of TBC1D10 subfamily genes in zebrafish

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns Pub Date : 2022-03-01 DOI:10.1016/j.gep.2021.119226

Shuna Sun , Ziyin Liu , Qiu Jiang , Yunzeng Zou

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引用次数: 0

Abstract

TBC1D10 subfamily has three members TBC1D10A-C, with the physiological and pathological functions such as melanosome transport, exosome secretion, and T-cell activation. However, the gene expression patterns and functions of this subfamily during embryonic development remain mysterious. In this study, we took advantage of zebrafish model to elucidate the spatial and temporal expression patterns of TBC1D10 subfamily genes including tbc1d10aa, tbc1d10ab, tbc1d10b, and tbc1d10c. Whole-mount in situ hybridization results showed robust tbc1d10aa expression and faint tbc1d10b expression as maternal transcripts except tbc1d10ab and tbc1d10c. In addition to pectoral fins, otic vesicles, and pharyngeal arch tissues, varying degrees of expression of all four subfamily members were observed in brain tissues and eyes (retinal inner nuclear layer). Besides, tbc1d10ab exhibited unique and enriched expression in the developing liver. Despite genetic conservativeness, all four members of zebrafish TBC1D10 subfamily shared several similarities and exhibited some distinctions in the expression patterns, indicating that they might have different and exclusive functions to be further explored.

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斑马鱼TBC1D10亚家族基因的胚胎表达模式

TBC1D10亚家族有三个成员TBC1D10A-C，具有黑素体转运、外泌体分泌、t细胞活化等生理病理功能。然而，这个亚家族在胚胎发育过程中的基因表达模式和功能仍然是一个谜。本研究利用斑马鱼模型，对TBC1D10亚家族基因tbc1d10aa、tbc1d10ab、tbc1d10b、tbc1d10c的时空表达模式进行了研究。全安装原位杂交结果显示，除tbc1d10ab和tbc1d10c外，母体转录本tbc1d10aa表达强劲，tbc1d10b表达微弱。除胸鳍、耳小泡和咽弓组织外，在脑组织和眼睛(视网膜内核层)均观察到不同程度的表达。此外，tbc1d10ab在发育中的肝脏中表现出独特且丰富的表达。尽管具有遗传保守性，但斑马鱼TBC1D10亚家族的4个成员在表达模式上有一些相似之处，并表现出一定的差异，这表明它们可能具有不同且排他的功能，有待进一步探索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation