Early Detection of Leukoplakic Oral Squamous Cell Carcinoma Using 4NQO-induced Rat Tongue Cancer Model: Study Utilizing Fluorescence Intensity and Histopathological Evaluation.

IF 0.5 Q4 DENTISTRY, ORAL SURGERY & MEDICINE
Bulletin of Tokyo Dental College Pub Date : 2022-03-08 Epub Date: 2022-02-15 DOI:10.2209/tdcpublication.2020-0014
Haruka Masuda, Nobuharu Yamamoto, Takahiko Shibahara
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引用次数: 2

Abstract

Early identification of leukoplakic oral squamous cell carcinoma (OSCC) is difficult. The purpose of this study was to determine whether it was possible to detect change from normal epithelium to leukoplakic OSCC using a fluorescence visualization (FV) device in a 4-nitroquinoline 1-oxide (4NQO) -induced rat tongue cancer model. If successful, this would facilitate early detection of OSCC. The rats (3 groups of 5) were administered 50 ppm 4NQO in their drinking water over a period of 10, 15, or 20 weeks. Five non-treated rats were used as a control group. Images of their tongues obtained by FV were analyzed for change in fluorescence intensity (FI) using image analysis software. Immunoreaction for anti-CK13, anti-CK17, and anti-E-cadherin antibodies was also histopathologically evaluated. Receiver operating characteristic (ROC) analysis was used to calculate the cut-off values, sensitivity, specificity, and area under the curve. The most marked change in FI was found between the control and 10-week groups, with an increase observed in its average value and range in the latter. These findings differed from those characteristic of leukoplakia. No significant difference was observed in the positive cell rate for immunoreaction for anti-CK13 or anti-CK17 antibodies between the control and 10-week groups. A significant decrease was observed in the positive pixel ratio of immunoreaction for anti-E-cadherin antibody in the 10-week group in comparison with in the control group (p <0.05). These results showed that disruption of intercellular adhesion could be observed at 10 weeks. In the ROC analysis, the FI cut-off value in the 10-week and control groups was 51.9, sensitivity 95.5%, and specificity 96.9%. This indicated that normal epithelium could be accurately distinguished from low-grade dysplasia with high probability. These results demonstrate that analysis of change in FI as measured by FV could facilitate early detection of leukoplakic OSCC.

4nqo诱导大鼠舌癌模型早期检测口腔白斑鳞状细胞癌的荧光强度及组织病理学评价研究
口腔白斑鳞状细胞癌(OSCC)的早期诊断是困难的。本研究的目的是确定在4-硝基喹啉- 1-氧化物(4NQO)诱导的大鼠舌癌模型中,荧光可视化(FV)装置是否有可能检测到正常上皮向白质鳞状细胞癌的变化。如果成功,这将有助于早期发现OSCC。大鼠(3组,每组5只)在10、15或20周的时间内,在其饮用水中添加50 ppm的4NQO。5只未治疗大鼠作为对照组。利用图像分析软件分析FV法获得的舌部荧光强度(FI)变化。抗ck13、抗ck17和抗e -cadherin抗体的免疫反应也进行了组织病理学评估。采用受试者工作特征(ROC)分析计算截止值、敏感性、特异性和曲线下面积。在对照组和10周组之间,FI的变化最为显著,后者的平均值和范围都有所增加。这些发现不同于白斑的特征。抗ck13或抗ck17抗体免疫反应阳性细胞率在对照组和10周组之间无显著差异。与对照组相比,治疗10周组抗e -钙粘蛋白抗体免疫反应阳性像元率显著降低(p
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来源期刊
Bulletin of Tokyo Dental College
Bulletin of Tokyo Dental College DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
0.90
自引率
0.00%
发文量
15
期刊介绍: The bulletin of Tokyo Dental collegue is principally for the publication of original contributions to multidisciplinary research in dentistry.
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