The Early Stages of Implantation and Placentation in the Pig.

Abstract

Pregnancy in pigs includes the events of conceptus (embryo/fetus and placental membranes) elongation, implantation, and placentation. Placentation in pigs is defined microscopically as epitheliochorial and macroscopically as diffuse. In general, placentation can be defined as the juxtapositioning of the endometrial/uterine microvasculature to the chorioallantoic/placental microvasculature to facilitate the transport of nutrients from the mother to the fetus to support fetal development and growth. Establishment of epitheliochorial placentation in the pig is achieved by: (1) the secretions of uterine glands prior to conceptus attachment to the uterus; (2) the development of extensive folding of the uterine-placental interface to maximize the surface area for movement of nutrients across this surface; (3) increased angiogenesis of the vasculature that delivers both uterine and placental blood and, with it, nutrients to this interface; (4) the minimization of connective tissue that lies between these blood vessels and the uterine and placental epithelia; (5) interdigitation of microvilli between the uterine and placental epithelia; and (6) the secretions of the uterine glands, called histotroph, that accumulate in areolae for transport though the placenta to the fetus. Placentation in pigs is not achieved by invasive growth of the placenta into the uterus. In this chapter, we summarize current knowledge about the major events that occur during the early stages of implantation and placentation in the pig. We will focus on the microanatomy of porcine placentation that builds off the excellent histological work of Amoroso and others and provide a brief review of some of the key physiological, cellular, and molecular events that accompany the development of "implantation" in pigs.