Metabolomic profiling of ZrO2 nanoparticles in MC3T3-E1 cells

IF 3.8 4区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

IET nanobiotechnology Pub Date : 2021-08-28 DOI:10.1049/nbt2.12067

Mingfu Ye, Linhu Wang, Zhang Wu, Wenjun Liu

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引用次数: 0

Abstract

The authors' previous study showed that zirconium oxide nanoparticles (ZrO₂ NPs) induce toxic effects in MC3T3-E1 cells; however, its toxicological mechanism is still unclear. Liquid chromatography–mass spectrometry/time-of-flight mass spectrometry was used to reveal the metabolite profile and toxicological mechanism of MC3T3-E1 cells in response to ZrO₂ NPs. The results demonstrated that MC3T3-E1 cells treated with ZrO₂ NPs for 24 and 48 h presented different metabolic characteristics. Following ZrO₂ NP treatment for 24 h, 96 upregulated and 129 downregulated metabolites in the positive ion mode, as well as 91 upregulated and 326 downregulated metabolites in the negative ion mode were identified. Following ZrO₂ NP treatment for 48 h, 33 upregulated and 174 downregulated metabolites were identified in the positive ion mode, whereas 37 upregulated and 302 downregulated metabolites were confirmed in the negative ion mode. Among them, 42 differential metabolites were recognised as potential metabolites contributing to the induced toxic effects of ZrO₂ NPs in MC3T3-E1 cells. Most of the differential metabolites were lysophosphatidylcholine and lysophosphatidylethanolamide, indicating that exposure to ZrO₂ NPs may have a profound impact on human cellular function by impairing the membrane system. The results also provide new clues for the toxicological mechanism of ZrO₂ NP dental materials.

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ZrO2纳米颗粒在MC3T3-E1细胞中的代谢组学分析

作者先前的研究表明，氧化锆纳米颗粒(ZrO2 NPs)对MC3T3-E1细胞具有毒性作用;然而，其毒理学机制尚不清楚。采用液相色谱-质谱/飞行时间质谱法研究MC3T3-E1细胞对ZrO2 NPs的代谢谱及毒理学机制。结果表明，ZrO2 NPs作用24和48 h的MC3T3-E1细胞代谢特征不同。ZrO2 NP处理24 h后，鉴定出正离子模式下96个代谢产物上调，129个代谢产物下调，负离子模式下91个代谢产物上调，326个代谢产物下调。ZrO2 NP处理48 h后，在正离子模式下鉴定出33个上调代谢物，174个下调代谢物，在负离子模式下鉴定出37个上调代谢物，302个下调代谢物。其中，42种差异代谢物被认为是参与ZrO2 NPs诱导MC3T3-E1细胞毒性作用的潜在代谢物。大多数差异代谢物是溶血磷脂酰胆碱和溶血磷脂酰乙醇酰胺，表明暴露于ZrO2 NPs可能通过损害膜系统对人体细胞功能产生深远影响。研究结果也为zro2np牙用材料的毒理学机制提供了新的线索。

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来源期刊

IET nanobiotechnology 工程技术-纳米科技

CiteScore

6.20

自引率

4.30%

发文量

审稿时长

1 months

期刊介绍： Electrical and electronic engineers have a long and illustrious history of contributing new theories and technologies to the biomedical sciences. This includes the cable theory for understanding the transmission of electrical signals in nerve axons and muscle fibres; dielectric techniques that advanced the understanding of cell membrane structures and membrane ion channels; electron and atomic force microscopy for investigating cells at the molecular level. Other engineering disciplines, along with contributions from the biological, chemical, materials and physical sciences, continue to provide groundbreaking contributions to this subject at the molecular and submolecular level. Our subject now extends from single molecule measurements using scanning probe techniques, through to interactions between cells and microstructures, micro- and nano-fluidics, and aspects of lab-on-chip technologies. The primary aim of IET Nanobiotechnology is to provide a vital resource for academic and industrial researchers operating in this exciting cross-disciplinary activity. We can only achieve this by publishing cutting edge research papers and expert review articles from the international engineering and scientific community. To attract such contributions we will exercise a commitment to our authors by ensuring that their manuscripts receive rapid constructive peer opinions and feedback across interdisciplinary boundaries. IET Nanobiotechnology covers all aspects of research and emerging technologies including, but not limited to: Fundamental theories and concepts applied to biomedical-related devices and methods at the micro- and nano-scale (including methods that employ electrokinetic, electrohydrodynamic, and optical trapping techniques) Micromachining and microfabrication tools and techniques applied to the top-down approach to nanobiotechnology Nanomachining and nanofabrication tools and techniques directed towards biomedical and biotechnological applications (e.g. applications of atomic force microscopy, scanning probe microscopy and related tools) Colloid chemistry applied to nanobiotechnology (e.g. cosmetics, suntan lotions, bio-active nanoparticles) Biosynthesis (also known as green synthesis) of nanoparticles; to be considered for publication, research papers in this area must be directed principally towards biomedical research and especially if they encompass in vivo models or proofs of concept. We welcome papers that are application-orientated or offer new concepts of substantial biomedical importance Techniques for probing cell physiology, cell adhesion sites and cell-cell communication Molecular self-assembly, including concepts of supramolecular chemistry, molecular recognition, and DNA nanotechnology Societal issues such as health and the environment Special issues. Call for papers: Smart Nanobiosensors for Next-generation Biomedical Applications - https://digital-library.theiet.org/files/IET_NBT_CFP_SNNBA.pdf Selected extended papers from the International conference of the 19th Asian BioCeramic Symposium - https://digital-library.theiet.org/files/IET_NBT_CFP_ABS.pdf