Urinary IgG, serum CX3CL1 and miRNA-152-3p: as predictors of nephropathy in Egyptian type 2 diabetic patients.

IF 3.6 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Tissue Barriers Pub Date : 2022-07-03 Epub Date: 2021-10-23 DOI:10.1080/21688370.2021.1994823
Aml E Abdou, Haneya A A Anani, Hanan F Ibrahim, Eman Elshohat Ebrahem, Nora Seliem, Eman M I Youssef, Niveen M Ghoraba, Asmaa S Hassan, Marwa A A Ramadan, Eman Mahmoud, Shorouk Issa, Hend M Maghraby, Eman K Abdelrahman, Hala Ali Mohammed Hassan
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引用次数: 4

Abstract

The purpose of this study was to assess the role of urinary IgG, serum CX3CL1 and miRNA 152-3p levels as predictors of nephropathy in type 2 Egyptian diabetic patients. Sixty type 2 diabetic patients and twenty healthy controls were enrolled in a cross-sectional study. Then they were grouped into: three groups based upon urine albumin excretion (UAE). The expression of miRNA 152-3p in serum was measured using quantitative polymerase chain reaction (RTq-PCR). Serum CX3CL1 and urinary IgG concentrations were measured by ELISA. RTq-PCR revealed that serum miRNA-152-3p levels in patients were significantly higher than in controls. There was significant differences between group with normoalbuminuria and groups with diabetic nephropathy DN as regard to age, duration of nephropathy, Albumin/Creatinine ratio (A/C ratio), creatinine, urine IgG, CX3CL1 and HbA1c. In diabetic patients, there was a significant positive correlation between miRNA-152-3p levels and disease duration only as well as significant positive correlations between urinary IgG levels and age, disease duration, serum creatinine, A/C ratio, and urea. Positive correlation between serum fractalkine CX3CL1 level and age, duration of disease, urea, creatinine, A/C ratio, HbA1C and IgG in patient with DN. Serum CX3CL1 level, urinary IgG were significantly increased with the progress of nephropathy so these integrated biomarkers could be used as good predictors for early identification of nephropathy. But miRNA- 152-3p has inadequate prognostic indicator for ESRD progression.

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尿IgG、血清CX3CL1和miRNA-152-3p:作为埃及2型糖尿病患者肾病的预测因子
本研究的目的是评估尿IgG、血清CX3CL1和miRNA 152-3p水平作为2型埃及糖尿病患者肾病的预测因子的作用。60名2型糖尿病患者和20名健康对照者参加了一项横断面研究。然后根据尿白蛋白排泄量(UAE)分为三组。采用定量聚合酶链反应(RTq-PCR)检测血清中miRNA 152-3p的表达。ELISA法检测血清CX3CL1和尿IgG浓度。RTq-PCR结果显示,患者血清miRNA-152-3p水平明显高于对照组。尿白蛋白正常组与糖尿病肾病DN组在年龄、肾病病程、白蛋白/肌酐比(A/C比)、肌酐、尿IgG、CX3CL1、HbA1c方面差异均有统计学意义。在糖尿病患者中,miRNA-152-3p水平仅与病程呈正相关,尿IgG水平与年龄、病程、血清肌酐、a /C比、尿素呈正相关。血清fractalkine CX3CL1水平与DN患者年龄、病程、尿素、肌酐、A/C比、HbA1C、IgG呈正相关。随着肾病的进展,血清CX3CL1水平、尿IgG水平显著升高,这些综合生物标志物可作为肾病早期识别的良好预测指标。但miRNA- 152-3p不能作为ESRD进展的预后指标。
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来源期刊
Tissue Barriers
Tissue Barriers MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.60
自引率
6.50%
发文量
25
期刊介绍: Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.
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