Virus Structures and Dynamics by Magic-Angle Spinning NMR.

IF 8.1 1区 医学 Q1 VIROLOGY
Gal Porat-Dahlerbruch, Amir Goldbourt, Tatyana Polenova
{"title":"Virus Structures and Dynamics by Magic-Angle Spinning NMR.","authors":"Gal Porat-Dahlerbruch,&nbsp;Amir Goldbourt,&nbsp;Tatyana Polenova","doi":"10.1146/annurev-virology-011921-064653","DOIUrl":null,"url":null,"abstract":"<p><p>Techniques for atomic-resolution structural biology have evolved during the past several decades. Breakthroughs in instrumentation, sample preparation, and data analysis that occurred in the past decade have enabled characterization of viruses with an unprecedented level of detail. Here we review the recent advances in magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy for structural analysis of viruses and viral assemblies. MAS NMR is a powerful method that yields information on 3D structures and dynamics in a broad range of experimental conditions. After a brief introduction, we discuss recent structural and functional studies of several viruses investigated with atomic resolution at various levels of structural organization, from individual domains of a membrane protein reconstituted into lipid bilayers to virus-like particles and intact viruses. We present examples of the unique information revealed by MAS NMR about drug binding, conduction mechanisms, interactions with cellular host factors, and DNA packaging in biologically relevant environments that are inaccessible by other methods.</p>","PeriodicalId":48761,"journal":{"name":"Annual Review of Virology","volume":"8 1","pages":"219-237"},"PeriodicalIF":8.1000,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8973440/pdf/nihms-1790240.pdf","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annual Review of Virology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1146/annurev-virology-011921-064653","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 11

Abstract

Techniques for atomic-resolution structural biology have evolved during the past several decades. Breakthroughs in instrumentation, sample preparation, and data analysis that occurred in the past decade have enabled characterization of viruses with an unprecedented level of detail. Here we review the recent advances in magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy for structural analysis of viruses and viral assemblies. MAS NMR is a powerful method that yields information on 3D structures and dynamics in a broad range of experimental conditions. After a brief introduction, we discuss recent structural and functional studies of several viruses investigated with atomic resolution at various levels of structural organization, from individual domains of a membrane protein reconstituted into lipid bilayers to virus-like particles and intact viruses. We present examples of the unique information revealed by MAS NMR about drug binding, conduction mechanisms, interactions with cellular host factors, and DNA packaging in biologically relevant environments that are inaccessible by other methods.

Abstract Image

魔角旋转核磁共振病毒结构和动力学。
原子分辨率结构生物学的技术在过去几十年中不断发展。在过去十年中,在仪器、样品制备和数据分析方面取得的突破使病毒的表征达到了前所未有的详细水平。本文综述了魔角自旋(MAS)核磁共振(NMR)技术在病毒及其组装体结构分析中的最新进展。MAS NMR是一种强大的方法,可以在广泛的实验条件下产生3D结构和动力学信息。在简要介绍之后,我们讨论了最近在不同结构组织水平上用原子分辨率研究的几种病毒的结构和功能研究,从膜蛋白的单个结构域重组为脂质双层到病毒样颗粒和完整病毒。我们展示了MAS NMR揭示的关于药物结合、传导机制、与细胞宿主因子的相互作用以及生物相关环境中DNA包装的独特信息,这些信息是其他方法无法获得的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
19.40
自引率
0.90%
发文量
28
期刊介绍: The Annual Review of Virology serves as a conduit for disseminating thrilling advancements in our comprehension of viruses spanning animals, plants, bacteria, archaea, fungi, and protozoa. Its reviews illuminate novel concepts and trajectories in basic virology, elucidating viral disease mechanisms, exploring virus-host interactions, and scrutinizing cellular and immune responses to virus infection. These reviews underscore the exceptional capacity of viruses as potent probes for investigating cellular function.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信