Endoglin promotes cell migration and invasion in endometriosis by regulating EMT.

IF 1.2 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Ginekologia polska Pub Date : 2024-01-01 Epub Date: 2021-09-20 DOI:10.5603/GP.a2021.0130

Xiutang Chen, Junjian Wang, Feixia Tu, Qing Yang, Dan Wang, Qin Zhu

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引用次数: 0

Abstract

Objectives: This study aims to investigate the expression and role of Endoglin (ENG) in endometriosis (EM).

Material and methods: In this study, quantitative real-time polymerase chain reaction, western blot and immunohistochemistry were used to examine the expression of ENG in tissues. Cellular experiments were performed to evaluate the effect of ENG on cellular biological function. Western blot was used to examine the expression of epithelial to mesenchymal transition-related proteins.

Results: The expression of ENG was significantly higher in the ectopic endometriotic tissues than that in eutopic endometriotic tissues. Knockdown of ENG inhibited cell viability, migration and invasion, and induced cell apoptosis in hEM15A cells. Additionally, silenced ENG caused increased levels of E-cadherin and decreased levels of N-cadherin, vimentin, MMP-2 and MMP-9.

Conclusions: These results confirmed that ENG may be involved in the development of endometriosis by promoting EMT process, revealing a new insight into the pathogenesis of endometriosis and contributing to the exploration of molecular therapeutic strategies against endometriosis.

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Endoglin 通过调节 EMT 促进子宫内膜异位症的细胞迁移和侵袭。

研究目的本研究旨在探讨子宫内膜异位症（EM）中Endoglin（ENG）的表达和作用：本研究采用实时定量聚合酶链反应、Western 印迹和免疫组织化学方法检测ENG在组织中的表达。细胞实验用于评估ENG对细胞生物功能的影响。Western 印迹用于检测上皮细胞向间充质转化相关蛋白的表达：结果：异位子宫内膜异位组织中ENG的表达明显高于异位子宫内膜异位组织。在 hEM15A 细胞中，敲除 ENG 可抑制细胞活力、迁移和侵袭，并诱导细胞凋亡。此外，沉默的ENG导致E-cadherin水平升高，N-cadherin、波形蛋白、MMP-2和MMP-9水平降低：这些结果证实了ENG可能通过促进EMT过程参与了子宫内膜异位症的发展，揭示了子宫内膜异位症发病机制的新见解，有助于探索子宫内膜异位症的分子治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ginekologia polska OBSTETRICS & GYNECOLOGY-

CiteScore

2.00

自引率

15.40%

发文量

317

审稿时长

4-8 weeks

期刊介绍： Ginekologia Polska’ is a monthly medical journal published in Polish and English language. ‘Ginekologia Polska’ will accept submissions relating to any aspect of gynaecology, obstetrics and areas directly related. ‘Ginekologia Polska’ publishes original contributions, comparative works, case studies, letters to the editor and many other categories of articles.