Monophasic Pulsed Current Stimulation of Duty Cycle 10% Promotes Differentiation of Human Dermal Fibroblasts into Myofibroblasts.

Physical therapy research Pub Date : 2021-03-18 eCollection Date: 2021-01-01 DOI:10.1298/ptr.E10064

Mikiko Uemura, Masaharu Sugimoto, Yoshiyuki Yoshikawa, Terutaka Hiramatsu, Taketo Inoue

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Abstract

Objective: Many clinical trials have shown the therapeutic effects of electrical stimulation (ES) in various conditions. Our previous studies showed that ES (200 μA and 2 Hz) promotes migration and proliferation of human dermal fibroblasts (HDFs). However, the effective duty cycle and the effect of ES on myofibroblast differentiation are unclear. This study aimed to investigate the relationship between duty cycle and myofibroblast differentiation.

Methods: HDFs were subjected to ES (200 μA and 2 Hz) for 24 h with the duty cycle adapted at 0% (control), 10%, 50%, or 90%. α-smooth muscle actin (SMA) and transforming growth factor (TGF)-β1 mRNA and α-SMA protein expressions were assessed. Collagen gel contraction was observed for 48 h after ES initiation and the gel area was measured. Cell viability and pH of culture medium were analyzed for cytotoxicity of the ES.

Results: Cell viabilities were decreased in the 50% and the 90% groups but ES did not influence on pH of culture media. ES with a duty cycle of 10% significantly promoted the mRNA expression of α-SMA and TGF-β1. α-SMA protein expression in the 10% group was also significantly higher than that of the control group. Collagen gel subjected to ES with a duty cycle of 10% was contracted.

Conclusion: Duty cycle can influence on myofibroblast differentiation and ES with a duty cycle 10% is the effective for wound healing.

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占空比为 10%的单相脉冲电流刺激可促进人真皮成纤维细胞分化为肌成纤维细胞

目的：许多临床试验表明，电刺激（ES）对各种疾病都有治疗作用。我们之前的研究表明，电刺激（200 μA 和 2 Hz）可促进人真皮成纤维细胞（HDFs）的迁移和增殖。然而，ES的有效占空比和对肌成纤维细胞分化的影响尚不清楚。本研究旨在探讨占空比与肌成纤维细胞分化之间的关系。方法：将 HDFs 放在 ES（200 μA 和 2 Hz）中 24 小时，占空比调整为 0%（对照组）、10%、50% 或 90%。在 ES 启动后的 48 小时内观察胶原凝胶收缩并测量凝胶面积。对细胞活力和培养基 pH 值进行分析，以确定 ES 的细胞毒性：结果：50% 和 90% 组的细胞活力下降，但 ES 对培养基的 pH 值没有影响。占空比为 10%的 ES 能明显促进 α-SMA 和 TGF-β1 的 mRNA 表达，10% 组的α-SMA 蛋白表达也明显高于对照组。在占空比为 10%的 ES 条件下，胶原凝胶发生收缩：占空比可影响肌成纤维细胞的分化，占空比为 10%的 ES 对伤口愈合有效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Physical therapy research

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