Perfecting Targeting in CRISPR.

IF 8.6 1区生物学 Q1 GENETICS & HEREDITY

Annual review of genetics Pub Date : 2021-11-23 Epub Date: 2021-09-16 DOI:10.1146/annurev-genet-071719-030438

Hainan Zhang, Tong Li, Yidi Sun, Hui Yang

引用次数: 7

Abstract

CRISPR-based genome editing holds promise for genome engineering and other applications in diverse organisms. Defining and improving the genome-wide and transcriptome-wide specificities of these editing tools are essential for realizing their full potential in basic research and biomedical therapeutics. This review provides an overview of CRISPR-based DNA- and RNA-editing technologies, methods to quantify their specificities, and key solutions to reduce off-target effects for research and improve therapeutic applications.

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完善CRISPR靶向技术。

基于crispr的基因组编辑为基因组工程和在不同生物体中的其他应用带来了希望。定义和改进这些编辑工具的全基因组和转录组特异性对于实现它们在基础研究和生物医学治疗中的全部潜力至关重要。本文综述了基于crispr的DNA和rna编辑技术，量化其特异性的方法，以及减少脱靶效应的研究和改善治疗应用的关键解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annual review of genetics 生物-遗传学

CiteScore

18.30

自引率

0.90%

发文量

期刊介绍： The Annual Review of Genetics, published since 1967, comprehensively covers significant advancements in genetics. It encompasses various areas such as biochemical, behavioral, cell, and developmental genetics, evolutionary and population genetics, chromosome structure and transmission, gene function and expression, mutation and repair, genomics, immunogenetics, and other topics related to the genetics of viruses, bacteria, fungi, plants, animals, and humans.