Characterization, enzymatic activity and biofilm formation of Candida species isolated from goat milk

IF 1.5 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia Pub Date : 2021-10-01 DOI:10.1016/j.riam.2021.06.001

Carolina Segundo Zaragoza , Itzel López Ortiz , David Alejandro Contreras Caro del Castillo , Yesmín María Domínguez Hernández , Juan Antonio Rodríguez García

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引用次数: 2

Abstract

Background

Data regarding yeast microbiota in goat milk is scarce.

Aims

To isolate and identify species of the genus Candida in milk samples from clinically healthy goats, and evaluate their enzymatic activity and biofilm formation.

Methods

1092 milk samples from clinically healthy goats were collected and processed. The yeast isolates were identified by phenotypic, methods and their enzymatic activity (phospholipase, hemolysin and protease) and biofilm formation evaluated.

Results

We obtained 221 Candida isolates belonging to six species: Candida kefyr (35.7%), Candida guilliermondii (33%), Candida famata (23.5%), Candida glabrata (5.9%), Candida albicans (1.35%) and Candida parapsilosis sensu lato (0.45%). Protease activity was detected in all Candida species while hemolysin activity was only present in C. kefyr, C. guilliermondii, C. famata and C. albicans. Only C. albicans showed phospholipase activity. With the exception of C. parapsilosis sensu lato, all Candida species formed biofilm, with 60.19% of the isolates being poor producers, 9.93% moderate producers, and 1.35% strong producers.

Conclusions

The milk of clinically healthy goats contains several species of the genus Candida that could play a role as opportunistic pathogens in mastitis.

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从羊奶中分离的念珠菌的鉴定、酶活性和生物膜形成

关于羊奶中酵母菌群的数据很少。目的分离鉴定临床健康山羊乳样品中的念珠菌属，并对其酶活性和生物膜形成进行评价。方法采集临床健康山羊羊奶样品1092份，并进行处理。对分离的酵母菌进行表型、方法鉴定，并对其酶活性(磷脂酶、溶血酶和蛋白酶)和生物膜形成进行评价。结果共分离念珠菌221株，分属6种:关键念珠菌(35.7%)、吉列mondii念珠菌(33%)、famata念珠菌(23.5%)、光秃念珠菌(5.9%)、白色念珠菌(1.35%)和敏感副念珠菌(0.45%)。蛋白酶在所有念珠菌中均有活性，溶血素仅在C. kefyr、C. guilliermondii、C. famata和C. albicans中有活性。只有白色念珠菌显示磷脂酶活性。除假丝酵母菌外，所有念珠菌均形成生物膜，60.19%为弱产菌，9.93%为中等产菌，1.35%为强产菌。结论临床健康山羊乳汁中含有多种念珠菌属，可能是乳腺炎的条件致病菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Iberoamericana De Micologia MYCOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

81 days

期刊介绍： Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.