Changing Gly311 to an acidic amino acid in the MATE family protein DTX6 enhances Arabidopsis resistance to the dihydropyridine herbicides.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular Plant Pub Date : 2021-12-06 Epub Date: 2021-09-10 DOI:10.1016/j.molp.2021.09.002
Zeyu Lv, Mingming Zhao, Wenjing Wang, Qi Wang, Mengqi Huang, Chaoqun Li, Qichao Lian, Jinqiu Xia, Ji Qi, Chengbin Xiang, Huiru Tang, Xiaochun Ge
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引用次数: 13

Abstract

In modern agriculture, frequent application of herbicides may induce the evolution of resistance in plants, but the mechanisms underlying herbicide resistance remain largely unexplored. Here, we report the characterization of rtp1 (resistant to paraquat 1), an Arabidopsis mutant showing strong resistance to the widely used herbicides paraquat and diquat. The rtp1 mutant is semi-dominant and carries a point mutation in the gene encoding the multidrug and toxic compound extrusion family protein DTX6, leading to the change of glycine to glutamic acid at residue 311 (G311E). The wild-type DTX6 with glycine 311 conferred weak paraquat and diquat resistance when overexpressed, while mutation of glycine 311 to a negatively charged amino acid (G311E or G311D) markedly increased the paraquat and diquat resistance of plants, whereas mutation to a positively charged amino acid (G311R or G311K) compromised the resistance, suggesting that the charge property of residue 311 of DTX6 is critical for the paraquat and diquat resistance of Arabidopsis plants. DTX6 is localized in the endomembrane trafficking system and may undergo the endosomal sorting to localize to the vacuole and plasma membrane. Treatment with the V-ATPase inhibitor ConA reduced the paraquat resistance of the rtp1 mutant. Paraquat release and uptake assays demonstrated that DTX6 is involved in both exocytosis and vacuolar sequestration of paraquat. DTX6 and DTX5 show functional redundancy as the dtx5 dtx6 double mutant but not the dtx6 single mutant plants were more sensitive to paraquat and diquat than the wild-type plants. Collectively, our work reveals a potential mechanism for the evolution of herbicide resistance in weeds and provides a promising gene for the manipulation of plant herbicide resistance.

将Gly311转变为MATE家族蛋白DTX6中的酸性氨基酸可增强拟南芥对二氢吡啶类除草剂的抗性。
在现代农业中,频繁使用除草剂可能诱导植物产生抗性,但其抗性机制仍未得到充分研究。在这里,我们报道了rtp1(抗百草枯1)的特性,这是一个拟南芥突变体,对广泛使用的除草剂百草枯和diquat具有很强的抗性。rtp1突变体为半显性突变体,在编码多药毒性化合物挤压家族蛋白DTX6的基因上携带一个点突变,导致甘氨酸在残基311处转变为谷氨酸(G311E)。带甘氨酸311的野生型DTX6在过表达时对百草枯和双甘菊的抗性较弱,而带负电荷的氨基酸(G311E或G311D)突变显著提高了植物对百草枯和双甘菊的抗性,而带正电荷的氨基酸(G311R或G311K)突变则降低了抗性,表明DTX6残基311的带电荷特性对拟南芥的百草枯和双甘菊抗性至关重要。DTX6定位于膜运输系统,可能经过内体分选定位于液泡和质膜。用v - atp酶抑制剂ConA治疗降低了rtp1突变体对百草枯的抗性。百草枯释放和吸收试验表明,DTX6参与了百草枯的胞吐和空泡隔离。DTX6和DTX5表现出功能冗余,DTX5双突变体比DTX6单突变体对百草枯和双甘菊更敏感。总的来说,我们的工作揭示了杂草抗除草剂进化的潜在机制,并为操纵植物抗除草剂提供了一个有希望的基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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