No hints for abundance of Bacillus anthracis and Burkholderia pseudomallei in 100 environmental samples from Cameroon.

Hagen Frickmann, Sven Poppert
{"title":"No hints for abundance of Bacillus anthracis and Burkholderia pseudomallei in 100 environmental samples from Cameroon.","authors":"Hagen Frickmann, Sven Poppert","doi":"10.1556/1886.2021.00014","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Little is known on the abundance of the pathogens Bacillus anthracis and Burkholderia pseudomallei in environmental samples in Cameroon. Therefore, 100 respective samples were assessed in a proof-of-principle assessment.</p><p><strong>Methods: </strong>DNA residuals from nucleic acid extractions of 100 environmental samples, which were collected between 2011 and 2013 in the Mapé Basin of Cameroon, were screened for B. anthracis and B. pseudomallei by real-time PCR. The samples comprised soil samples with water contact (n = 88), soil samples without water contact (n = 6), plant material with water contact (n = 3), water (n = 2), and soil from a hospital dressing room (n = 1).</p><p><strong>Results: </strong>B. anthracis and B. pseudomallei were detected in none of the samples assessed.</p><p><strong>Conclusion: </strong>The results indicate that at least a quantitatively overwhelming, ubiquitous occurrence of B. anthracis and B. pseudomallei in the environment in Cameroon is highly unlikely. However, the number and choice of the assessed samples limit the interpretability of the results.</p>","PeriodicalId":11929,"journal":{"name":"European Journal of Microbiology & Immunology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/16/61/eujmi-11-057.PMC8614492.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Microbiology & Immunology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1556/1886.2021.00014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Little is known on the abundance of the pathogens Bacillus anthracis and Burkholderia pseudomallei in environmental samples in Cameroon. Therefore, 100 respective samples were assessed in a proof-of-principle assessment.

Methods: DNA residuals from nucleic acid extractions of 100 environmental samples, which were collected between 2011 and 2013 in the Mapé Basin of Cameroon, were screened for B. anthracis and B. pseudomallei by real-time PCR. The samples comprised soil samples with water contact (n = 88), soil samples without water contact (n = 6), plant material with water contact (n = 3), water (n = 2), and soil from a hospital dressing room (n = 1).

Results: B. anthracis and B. pseudomallei were detected in none of the samples assessed.

Conclusion: The results indicate that at least a quantitatively overwhelming, ubiquitous occurrence of B. anthracis and B. pseudomallei in the environment in Cameroon is highly unlikely. However, the number and choice of the assessed samples limit the interpretability of the results.

Abstract Image

喀麦隆 100 份环境样本中炭疽杆菌和假马勒伯克霍尔德氏菌的数量没有提示。
背景:人们对喀麦隆环境样本中病原体炭疽杆菌和假马勒伯克霍尔德氏菌的含量知之甚少。因此,我们对 100 份样本进行了原则性评估:对 2011 年至 2013 年期间在喀麦隆马佩盆地采集的 100 份环境样本的核酸提取物中残留的 DNA 进行了实时 PCR 筛选,以检测炭疽杆菌和假马勒伯克霍尔德氏菌。这些样本包括与水接触的土壤样本(88 个)、未与水接触的土壤样本(6 个)、与水接触的植物材料(3 个)、水(2 个)以及来自医院更衣室的土壤(1 个):结果:在所有评估样本中均未检测到炭疽杆菌和假马来杆菌:结果表明,在喀麦隆的环境中,炭疽杆菌和假丝酵母菌至少在数量上不可能无处不在。然而,评估样本的数量和选择限制了结果的可解释性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信