Evaluation of the Effect of Pomegranate Seed Oil on Healing in a Rat Wound Model With Antioxidant, Vascular, and Histopathological Parameters.

IF 1.5 4区医学 Q3 DERMATOLOGY

International Journal of Lower Extremity Wounds Pub Date : 2023-12-01 Epub Date: 2021-08-31 DOI:10.1177/15347346211040593

A Nilhan Atsü Md, Zeynep Tosuner Md, Tayfun Bilgiç Md

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引用次数: 2

Abstract

The study aimed to evaluate the efficiency of pomegranate (Punica granatum) seed oil in wound healing in excised rats. Sixteen female young Wistar Albino Rats weighing approximately 300 to 320 g were randomly divided into 2 groups as the treatment (=pomegranate seed oil group) and control groups in this experiment. Six different wounds 1 cm apart from the midline and each other were formed with a 6 mm punch biopsy instrument. Three wounds were left open (open wound group) whereas 3 wounds were sutured with 4/0 vicryl (closed wound group). Punica granatum seed oil treatment was administered topically to the treatment group, both to open and closed wounds, once a day for 14 days. Parameters for healing were evaluated. Histopathologic examination was performed for the investigation of inflammation, neovascularization, granulation, and fibroblast generation in addition to serologic (enzyme-linked immunosorbent assay) evaluation of rat malondialchehyche, rat glutathione peroxidase, and rat superoxide dismutase. PeriScan PIM 3 System Laser Doppler Blood Perfusion Imager was used for the calculation of blood perfusion. There was a statistically significant difference between inflammation and neovascularization levels and group type on the 14th day in open wounds(P < .05). On the 21st day, the granulation tissue level in the closed wound group was found to be higher in the pomegranate group (P = 0.000).The results showed that PSE oil is partially effective, although it is not effective in every parameter examined, in the treatment of excised wounds in rats and may be suitable for clinical treatment in humans but large controlled studies are needed.

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评价石榴籽油对具有抗氧化、血管和组织病理学参数的大鼠伤口模型愈合的影响。

本研究旨在评价石榴籽油对离体大鼠伤口愈合的影响。16只雌性幼龄Wistar Albino大鼠，体重约300至320 g随机分为两组，分别为石榴籽油组和对照组。在距中线1cm处形成6个不同的伤口 mm打孔活检仪。三个伤口是开放的（开放伤口组），而三个伤口用4/0 vicryl缝合（闭合伤口组）。治疗组对开放性和闭合性伤口局部给予石榴籽油治疗，每天一次，持续14天。对愈合参数进行了评估。除了对大鼠丙二酸二乙酯、大鼠谷胱甘肽过氧化物酶和大鼠超氧化物歧化酶的血清学（酶联免疫吸附试验）评估外，还进行了组织病理学检查，以研究炎症、新生血管、肉芽和成纤维细胞的生成。PeriScan PIM 3系统激光多普勒血液灌注成像仪用于计算血液灌注。开放性创面第14天的炎症和新生血管生成水平与分组类型之间有统计学意义（P P = 0.000）。结果表明，PSE油在大鼠切除伤口的治疗中是部分有效的，尽管它在所检查的每个参数中都不是有效的，并且可能适用于人类的临床治疗，但需要进行大规模的对照研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Lower Extremity Wounds DERMATOLOGY-SURGERY

CiteScore

4.60

自引率

17.60%

发文量

审稿时长

>12 weeks

期刊介绍： The International Journal of Lower Extremity Wounds (IJLEW) is a quarterly, peer-reviewed journal publishing original research, reviews of evidence-based diagnostic techniques and methods, disease and patient management, and surgical and medical therapeutics for lower extremity wounds such as burns, stomas, ulcers, fistulas, and traumatic wounds. IJLEW also offers evaluations of assessment and monitoring tools, dressings, gels, cleansers, pressure management, footwear/orthotics, casting, and bioengineered skin. This journal is a member of the Committee on Publication Ethics (COPE).