Possible relationship between mitochondrial changes and oxidative stress under low dose-rate irradiation.

IF 5.2 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qingmei Meng, Elena Karamfilova Zaharieva, Megumi Sasatani, Junya Kobayashi
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引用次数: 0

Abstract

Objectives: High dose-rate ionizing radiation (IR) causes severe DSB damage, as well as reactive oxygen species (ROS) accumulation and oxidative stress. However, it is unknown what biological processes are affected by low dose-rate IR; therefore, the molecular relationships between mitochondria changes and oxidative stress in human normal cells was investigated after low dose-rate IR.Methods: We compared several cellular response between high and low dose-rate irradiation using cell survival assay, ROS/RNS assay, immunofluorescence and western blot analysis.Results: Reduced DSB damage and increased levels of ROS, with subsequent oxidative stress responses, were observed in normal cells after low dose-rate IR. Low dose-rate IR caused several mitochondrial changes, including morphology mass, and mitochondrial membrane potential, suggesting that mitochondrial damage was caused. Although damaged mitochondria were removed by mitophagy to stop ROS leakage, the mitophagy-regulatory factor, PINK1, was reduced following low dose-rate IR. Although mitochondrial dynamics (fission/fusion events) are important for the proper mitophagy process, some mitochondrial fusion factors decreased following low dose-rate IR.Discussion: The dysfunction of mitophagy pathway under low dose-rate IR increased ROS and the subsequent activation of the oxidative stress response.

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低剂量率辐照下线粒体变化与氧化应激之间的可能关系
目的:高剂量率电离辐射(IR)会造成严重的DSB损伤以及活性氧(ROS)积累和氧化应激。因此,我们研究了低剂量电离辐射后人体正常细胞线粒体变化与氧化应激之间的分子关系:方法:我们使用细胞存活测定、ROS/RNS测定、免疫荧光和Western印迹分析比较了高剂量率和低剂量率辐照对细胞的影响:结果:低剂量红外照射后,正常细胞中的DSB损伤减少,ROS水平升高,随后出现氧化应激反应。低剂量红外引起了线粒体的一些变化,包括形态质量和线粒体膜电位,表明线粒体受到了损伤。虽然受损线粒体被有丝分裂清除以阻止ROS泄漏,但有丝分裂调节因子PINK1在低剂量红外线照射后却减少了。虽然线粒体动力学(裂变/融合事件)对正常的有丝分裂过程很重要,但一些线粒体融合因子在低剂量红外照射后减少了:讨论:在低剂量红外条件下,有丝分裂途径的功能障碍增加了 ROS,从而激活了氧化应激反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Redox Report
Redox Report 生物-生化与分子生物学
CiteScore
6.10
自引率
0.00%
发文量
28
审稿时长
>12 weeks
期刊介绍: Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.
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