Presence of TSHR in NK Cells and Action of TSH on NK Cells.

IF 2.2 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Neuroimmunomodulation Pub Date : 2022-01-01 Epub Date: 2021-08-13 DOI:10.1159/000516925

Qingqing Yang, Jingyi Li, Chunjia Kou, Li Zhang, Xiansheng Wang, Yu Long, Jiajia Ni, Shuqi Li, Haiqing Zhang

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引用次数: 2

Abstract

Introduction: Thyroid-stimulating hormone receptor (TSHR) is widely expressed in human tissues and cells. TSHR is not only involved in thyroid disease but also in the neuroendocrine-immune regulatory network. However, no study has exclusively focused on the expression and function of TSHR in natural killer (NK) cells.

Methods: We studied TSHR expression using reverse transcription PCR to verify TSHR mRNA transcripts in human and mouse NK cells. Human and mouse thyroid and liver tissues as well as peripheral blood mononuclear cells (PBMCs) or spleen lymphoid cells (SLCs) were used as controls. The TSHR protein levels in NK-92 cells were determined by immunofluorescence staining. The function of TSHR in NK cells was investigated by measuring the TSH-stimulated cAMP levels.

Results: TSHR mRNA was detected in human and mouse NK cells as well as in NK-92 cells and had the same sequence as that of thyroid-derived, PBMC-derived, and liver-derived mRNA. The TSHR protein was also expressed in the cell membrane of NK-92 cells. Furthermore, the cAMP levels in NK-92 cells were significantly higher after adding 102 mIU/mL of bovine TSH at p < 0.05, which stimulated cAMP production in NK-92 cells.

Conclusions: Our findings confirm that TSHR is present and functional in NK cells and provide key clues for the potential regulatory effects of TSH on TSHR in NK cells in the immune system.

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NK细胞中TSHR的存在及其对NK细胞的作用。

促甲状腺激素受体(Thyroid-stimulating hormone receptor, TSHR)广泛表达于人体组织和细胞中。TSHR不仅参与甲状腺疾病，还参与神经内分泌免疫调节网络。然而，目前还没有专门研究TSHR在自然杀伤细胞(NK)中的表达和功能。方法:采用反转录PCR方法研究TSHR在人和小鼠NK细胞中的表达，验证TSHR mRNA转录物。以人和小鼠甲状腺和肝脏组织以及外周血单个核细胞(PBMCs)或脾脏淋巴样细胞(SLCs)作为对照。免疫荧光染色法检测NK-92细胞中TSHR蛋白水平。通过测量tsh刺激的cAMP水平，研究NK细胞TSHR的功能。结果:在人和小鼠NK细胞以及NK-92细胞中均检测到TSHR mRNA，其序列与甲状腺源性、pbmc源性和肝源性mRNA序列相同。NK-92细胞的细胞膜中也有TSHR蛋白的表达。添加102 mIU/mL牛TSH后，NK-92细胞cAMP水平显著升高(p < 0.05)，促进了NK-92细胞cAMP的生成。结论:我们的研究结果证实了TSHR在NK细胞中存在并起作用，并为TSH在免疫系统中对NK细胞中TSHR的潜在调节作用提供了关键线索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroimmunomodulation 医学-免疫学

CiteScore

3.60

自引率

4.20%

发文量

审稿时长

>12 weeks

期刊介绍： The rapidly expanding area of research known as neuroimmunomodulation explores the way in which the nervous system interacts with the immune system via neural, hormonal, and paracrine actions. Encompassing both basic and clinical research, ''Neuroimmunomodulation'' reports on all aspects of these interactions. Basic investigations consider all neural and humoral networks from molecular genetics through cell regulation to integrative systems of the body. The journal also aims to clarify the basic mechanisms involved in the pathogenesis of the CNS pathology in AIDS patients and in various neurodegenerative diseases. Although primarily devoted to research articles, timely reviews are published on a regular basis.