Potential of Autologous Progenitor Cells and Decellularized Porcine Artery Matrix in Construction of Tissue-engineered Vascular Grafts.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Organogenesis Pub Date : 2021-10-02 Epub Date: 2021-08-18 DOI:10.1080/15476278.2021.1963603

Jieh-Neng Wang, Chung-Dann Kan, Shao-Hsien Lin, Ko-Chi Chang, Stephanie Tsao, Tak-Wah Wong

引用次数: 0

Abstract

To develop a tissue-engineered vascular graft, we used pericardial effusion-derived progenitor cells (PEPCs) collected from drained fluid after open-heart surgery in children with congenital heart diseases to repopulate a decellularized porcine pulmonary artery. The PEPCs were compared with human fibroblasts (HS68) and human umbilical vein endothelial cells (HUVECs) in cell growth and migration. They were cultured with the matrices via an inner approach (intima), lateral approach (media), and outer approach (adventitia). PEPCs grew and migrated better than the other two cells 14 days after seeding in the decellularized vessel. In immunofluorescence assays, PEPCs expressed CD90 and CD105 indicating a vascular differentiation. PEPCs grew in a decellularized porcine pulmonary artery matrix may have the potential for producing tissue-engineered vascular grafts.

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自体祖细胞和脱细胞猪动脉基质在构建组织工程血管移植物中的潜力

为了开发一种组织工程血管移植物，我们利用从先天性心脏病患儿开胸手术后排出的液体中收集的心包积液衍生祖细胞（PEPCs）重新填充脱细胞猪肺动脉。在细胞生长和迁移方面，PEPCs 与人成纤维细胞（HS68）和人脐静脉内皮细胞（HUVECs）进行了比较。它们通过内侧（内膜）、外侧（中膜）和外侧（外膜）与基质一起培养。在脱细胞血管中播种 14 天后，PEPCs 的生长和迁移优于其他两种细胞。在免疫荧光检测中，PEPCs 表达了 CD90 和 CD105，表明其已向血管分化。在脱细胞猪肺动脉基质中生长的 PEPCs 可能具有制造组织工程血管移植物的潜力。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.