Detecting Pb²⁺by a 'turn-on' fluorescence sensor based on DNA functionalized magnetic nanocomposites.

IF 2.8 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nanotechnology Pub Date : 2021-11-24 DOI:10.1088/1361-6528/ac1dd3

Jiayi Li, Yu Jin, Yaoqiang Wang, Yilin Zhao, Haijia Su

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引用次数: 6

Abstract

Sensitive and selective detection of the lead ion (Pb²⁺) plays an important role in terms of both human health and environmental protection, as the heavy metal is fairly ubiquitous and highly toxic. The highly stable fluorescence biosensor is composed of Fe₃O₄@TiO₂core-shell nanocomposites, functionalized with a carboxyl fluorescein labeled DNA. The morphology, physical and chemical properties of the sensing nanomaterials were studied by transmission electron microscopy, FT-IR spectroscopy (FT-IR), x-ray powder diffraction and vibrating sample magnetometer. UV-visible and fluorescence spectroscopy were used to characterize the fluorescein functionalized magnetic nanoparticles. The performance of Pb²⁺detection displayed an excellent linearity (R² = 0.995) in the range of 10^-10to 5 × 10^-9ppm with a detection limit of 10^-10ppm, based on the optimization of the fabrication process and aptamers' specification. The fluorescence biosensor has an accurate response, excellent recoveries and high adsorbent capacities. It was successfully applied for the determination of Pb²⁺in contaminated water and serum samples; the detection of limit in both media were 10^-10ppm. These features ensure the potential use of aptamer functionalized magnetic nanocomposites as a new class of non-toxic biocompatible sensors for biological and environmental applications.

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基于DNA功能化磁性纳米复合材料的“开启”荧光传感器检测Pb2+。

铅离子(Pb2+)是一种普遍存在且具有高毒性的重金属，对其进行灵敏、选择性的检测对人类健康和环境保护具有重要意义。高度稳定的荧光生物传感器由Fe3O4@TiO2core-shell纳米复合材料组成，用羧基荧光素标记的DNA功能化。采用透射电子显微镜、红外光谱(FT-IR)、x射线粉末衍射和振动样品磁强计对传感纳米材料的形貌、理化性质进行了研究。利用紫外可见光谱和荧光光谱对荧光素功能化磁性纳米颗粒进行了表征。在10-10 ~ 5 × 10-9ppm范围内，对Pb2+的检测表现出良好的线性关系(R2 = 0.995)，检出限为10-10ppm。荧光生物传感器具有响应准确、回收率高、吸附能力强等特点。该方法成功地应用于污染水和血清样品中Pb2+的测定;两种介质的检出限均为10 ~ 10ppm。这些特性确保了适体功能化磁性纳米复合材料作为一种新型无毒生物相容性传感器的潜在用途，可用于生物和环境应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanotechnology 工程技术-材料科学：综合

CiteScore

7.10

自引率

5.70%

发文量

820

审稿时长

2.5 months

期刊介绍： The journal aims to publish papers at the forefront of nanoscale science and technology and especially those of an interdisciplinary nature. Here, nanotechnology is taken to include the ability to individually address, control, and modify structures, materials and devices with nanometre precision, and the synthesis of such structures into systems of micro- and macroscopic dimensions such as MEMS based devices. It encompasses the understanding of the fundamental physics, chemistry, biology and technology of nanometre-scale objects and how such objects can be used in the areas of computation, sensors, nanostructured materials and nano-biotechnology.