"Here we go again": the inspection of collagen extraction protocols for 14C dating and palaeodietary analysis.

IF 2.8 0 ARCHAEOLOGY
Sahra Talamo, Helen Fewlass, Raquel Maria, Klervia Jaouen
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引用次数: 25

Abstract

Archaeological bone collagen is highly useful for radiocarbon (14C) dating and palaeodietary reconstruction. However, collagen preservation and carbon contamination are essential considerations when extracting collagen, becoming especially crucial close to the limit of the method (50,000 years before present = BP). Strong progress has been achieved in the past two decades by 14C and stable isotopic laboratories in removing contamination from archaeological bones, but different pretreatment protocols have been proven to produce varying results. Here we compare three collagen extraction protocols used for palaeodietary studies and 14C dating, considering collagen yield, elemental and stable isotopic data, FTIR analysis, and 14C dates. We focus on the impact of ultrafiltration on the yield and quality of the extracted material. The results again underline the importance of rigorous decontamination methods to gain accurate 14C dates and demonstrate that different protocols have significant effects on the quality and yield of extracted collagen.

Abstract Image

Abstract Image

Abstract Image

“又来了”:检查胶原蛋白提取方案,用于14C测年和古饮食分析。
考古骨胶原对放射性碳(14C)测年和古饮食重建非常有用。然而,在提取胶原蛋白时,胶原蛋白的保存和碳污染是必不可少的考虑因素,在接近该方法的极限时(距今5万年= BP)变得尤为重要。在过去的二十年里,14C和稳定同位素实验室在去除考古骨骼中的污染方面取得了很大的进展,但不同的预处理方案已被证明产生不同的结果。在这里,我们比较了三种用于古饮食研究和14C测年的胶原提取方案,考虑了胶原产量、元素和稳定同位素数据、FTIR分析和14C测年。我们重点研究了超滤对萃取物收率和质量的影响。结果再次强调了严格的去污方法对获得准确的14C日期的重要性,并表明不同的方案对提取的胶原蛋白的质量和产量有显著影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.30
自引率
0.00%
发文量
2
审稿时长
6 weeks
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