Detection of aflatoxin producing Aspergillus flavus from animal feed in Karnataka, India.

Environmental analysis, health and toxicology Pub Date : 2021-09-01 Epub Date: 2021-07-27 DOI:10.5620/eaht.2021017

Pethannan Rajarajan, Katherin Sylvia, Malaiyarasa Pandian Periasamy, Maheswari Subramanian

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引用次数: 3

Abstract

Aflatoxins are toxic carcinogenic secondary metabolite produced by Aspergillus flavus and are responsible for contamination in animal feed. The aim of the study was to determine the prevalence of aflatoxin contamination in animal feed in Karnataka state, India. The screening was performed by desiccated coconut agar and quantification of aflatoxin by liquid ammonia vapor test, TLC and ELISA. A total of 29 samples received from different places of Karnataka were analysed for aflatoxin B1. Out of 29 animal feed sample aflatoxin B1 detected in 12 samples representing 41.38% at average concentration of 288.50 μg/kg. Out of 42 isolates screened in animal feed, Aspergillus flavus was found to be in 86.2% and Aspergillus niger was 24.1%. It was observed that out of 42 isolates analyzed from animal feed, aflatoxin B1 was detected in 12 samples. Aflatoxin B1 is the most common contaminant and the method is more sensitive in screening and detection of aflatoxin B1 in the animal feed.

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印度卡纳塔克邦动物饲料中产生黄曲霉的黄曲霉的检测。

黄曲霉毒素是由黄曲霉产生的有毒致癌次生代谢物，是造成动物饲料污染的主要原因。该研究的目的是确定印度卡纳塔克邦动物饲料中黄曲霉毒素污染的流行程度。采用干燥椰子琼脂进行筛选，液氨气法、薄层色谱法和酶联免疫吸附测定黄曲霉毒素含量。从卡纳塔克邦不同地方收到的29份样本进行了黄曲霉毒素B1分析。在29份动物饲料样品中，12份样品中黄曲霉毒素B1含量为41.38%，平均浓度为288.50 μg/kg。在动物饲料中筛选出的42株分离菌中，黄曲霉占86.2%，黑曲霉占24.1%。从饲料中分离出42株黄曲霉毒素，其中12株检出黄曲霉毒素B1。黄曲霉毒素B1是最常见的污染物，该方法对动物饲料中黄曲霉毒素B1的筛选和检测更为灵敏。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Environmental analysis, health and toxicology

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