Saliva nitrite is higher in male children with autism spectrum disorder and positively correlated with serum nitrate.

IF 5.2 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Lulu Yao, Huimin Fu, Lu Bai, Wenwen Deng, Fang Xie, Ying Li, Rong Zhang, Xinjie Xu, Ting Wang, Shenghan Lai, Jun Wang
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引用次数: 0

Abstract

Objectives: Nitric oxide (NO) plays a vital role in neurological development. As an easily accessible and non-invasive fluid, saliva hasn't been evaluated for nitrite among children with autism spectrum disorder (ASD). This study aims to quantify saliva nitrite and explore its relation with serum NO.

Methods: Saliva sampling and pretreatment methods were optimized, followed by NO measurement via chemiluminescence for 126 ASD children and 129 normally developing children (ND).

Results: In the ASD group, saliva nitrite was significantly higher than that in the ND, with concentrations of 4.97 ± 3.77 μM and 2.66 ± 2.07 μM (p < 0.0001), respectively. Positive correlation was observed between saliva NO2- and serum NO3- in ASD children, which didn't exist in the ND group. Male children in the ASD group had significantly higher NO than that in boys of the ND group, without significant difference between girls in both groups. Correlation was not found between saliva or serum NO and severity of these ASD children.

Discussion: It is reported for the first time that saliva nitrite was positively correlated with serum nitrate in ASD children, with significantly higher NO only in autistic boys. Non-invasive saliva might serve as a predictor of health status of ASD children.

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患有自闭症谱系障碍的男性儿童唾液中亚硝酸盐含量较高,且与血清硝酸盐呈正相关。
目的:一氧化氮(NO一氧化氮(NO)在神经系统发育中起着至关重要的作用。自闭症谱系障碍(ASD)儿童唾液中的亚硝酸盐容易获得且无创伤,但尚未对唾液中的亚硝酸盐进行评估。本研究旨在量化唾液中的亚硝酸盐,并探讨其与血清中 NO 的关系:方法:优化唾液采样和预处理方法,然后通过化学发光法测定126名自闭症谱系障碍儿童和129名发育正常儿童(ND)的NO:结果:在 ASD 组中,唾液中亚硝酸盐的浓度明显高于 ND 组,分别为 4.97 ± 3.77 μM 和 2.66 ± 2.07 μM(p < 0.0001)。ASD儿童唾液中的NO2-与血清中的NO3-呈正相关,而ND组儿童则不存在这种情况。ASD组男性儿童的NO2明显高于ND组的男性儿童,而两组女孩之间没有明显差异。唾液或血清中的 NO 与 ASD 儿童的严重程度之间没有相关性:讨论:这是首次报道自闭症儿童唾液中的亚硝酸盐与血清中的硝酸盐呈正相关,只有自闭症男孩的唾液中的氮氧化物显著较高。无创唾液可作为 ASD 儿童健康状况的预测指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Redox Report
Redox Report 生物-生化与分子生物学
CiteScore
6.10
自引率
0.00%
发文量
28
审稿时长
>12 weeks
期刊介绍: Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.
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