Detection of SARS-CoV-2 antibodies in pediatric patients: An Iranian referral hospital-based study.

Q3 Medicine

Human Antibodies Pub Date : 2021-01-01 DOI:10.3233/HAB-210448

Setareh Mamishi, Golnaz Ghazizadeh Esslami, Mohsen Mohammadi, Mohammad Reza Abdolsalehi, Reihaneh Hosseinpour Sadeghi, Shima Mahmoudi, Babak Pourakbari

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引用次数: 2

Abstract

Background: As the extent of the pandemic and its seroprevalence pattern has been less clarified in pediatrics to date, we aimed to conduct this study to investigate the clinical and laboratory characteristics of COVID-19 in Iranian children, with a focus on evaluating the antibody prevalence and its relation with the laboratory tests.

Methods: All children with highly suspected COVID-19 were included. Anti-nucleoprotein SARS-CoV-2 were measured using SARS-CoV-2 immunoglobulin M (IgM) and SARS-CoV-2 IgG ELISA kits. Hypothesis testing was carried out through SPSS to unravel any association between the measurement tools and important clinical and laboratory characteristics.

Results: In this study, 254 patients were evaluated and 117 cases (46%) were male. The nucleic acid detection results for patient 55 were negative, but the IgM and IgG results were positive. Totally, 190 patients were tested for IgM in which only 14 (7.3%) had positive tests. Positive IgG was detected in 51 (20%) out of 254 patients; among them, 30 patients had negative SARS-CoV-2 RT-PCR (59%). Lower level of platelets in IgG positive group in comparison with the IgG negative group was observed (P value: 0.015). Moreover, higher alanine aminotransferase (ALT) was observed in the in IgG positive group (P value: 0.02). In patients with positive IgM, relative hypocalcemia (median of 8.25; IQR: 8.02-8.62) was found which appeared to be significant (P value: 0.02).

Conclusion: This is the first largest study describing the SARS-CoV-2 seropositivity among children in Iran and provides important insight about the COVID-19 infection in children.

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儿童患者SARS-CoV-2抗体检测:一项基于伊朗转诊医院的研究

背景:由于迄今为止在儿科中大流行的程度及其血清阳性率模式尚不清楚，我们旨在开展本研究，调查伊朗儿童COVID-19的临床和实验室特征，重点评估抗体流行率及其与实验室检测的关系。方法:纳入所有高度疑似COVID-19患儿。采用SARS-CoV-2免疫球蛋白M (IgM)和SARS-CoV-2 IgG ELISA试剂盒检测SARS-CoV-2抗核蛋白水平。通过SPSS进行假设检验，以揭示测量工具与重要临床和实验室特征之间的任何关联。结果:本研究共评估254例患者，其中男性117例(46%)。55例患者核酸检测结果为阴性，但IgM和IgG结果为阳性。总共有190例患者接受了IgM检测，其中只有14例(7.3%)呈阳性。254例患者中IgG阳性51例(20%);其中SARS-CoV-2 RT-PCR阴性30例(59%)。IgG阳性组血小板水平低于IgG阴性组(P值:0.015)。IgG阳性组丙氨酸转氨酶(ALT)升高(P值:0.02)。在IgM阳性的患者中，相对低钙(中位数为8.25;IQR: 8.02-8.62)，差异有统计学意义(P值:0.02)。结论:这是首个描述伊朗儿童SARS-CoV-2血清阳性的大型研究，为了解儿童COVID-19感染提供了重要见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Antibodies Medicine-Immunology and Allergy

CiteScore

3.50

自引率

0.00%

发文量

期刊介绍： Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.