HER2 overexpression is a putative diagnostic and prognostic biomarker for late-stage colorectal cancer in North African patients.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-12-01 DOI:10.1080/19932820.2021.1955462

Eman A Abdul Razzaq, Thenmozhi Venkatachalam, Khuloud Bajbouj, Mohamed Rahmani, Amena Mahdami, Surendra Rawat, Naziha Mansuri, Hussein Alhashemi, Rifat Akram Hamoudi, Riyad Bendardaf

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引用次数: 7

Abstract

Aim: Colorectal cancer (CRC) is one of the leading cancers in the world. Even though its mortality and pathophysiology are well documented in the US and the European countries, it is seldom studied in North African population. Recent studies have shown link of HER2 overexpression in oesophageal and gastric cancers. The aim of this study is to assess the HER2 protein and mRNA expression and its correlation with tumor pathogenesis in Libyan CRC patients.Methodology: A total of 17 FFPE tissue blocks were collected from patients with primary CRC. The HER2 protein expression was assessed by immunohistochemistry and the mRNA expression was assessed using qRT-PCR. Survival analysis of the role of HER2 overexpression on rectal adenocarcinoma was carried out on additional 165 patients.Results: From the CRC cohort, adenocarcinoma was found to be more frequent accounting for 88.2%, and 11.8% for mucinous adenocarcinomas. Almost 47% of the cases were positive for HER2 (score ≥ 2+) and about 50% adenocarcinoma cases with tumor grade II were positive for HER2. Moreover, 57.4% adenocarcinoma patients with grade-II tumor had undergone right hemicolectomy. Furthermore, significant correlation (p = 0.03) between the HER2 mRNA expression with the tumor grade was observed. In addition, poor overall all survival was observed with high HER2 expression in rectum adenocarcinoma.Conclusion: To our knowledge, this is the first study that HER2 overexpression correlates with more aggressive colorectal cancer in North African population. Our study shows that HER2 overexpression associates with right colon surgeries. Also, the correlation of mRNA and protein expression could warrant the implementation of a nationwide screening program for HER2 positivity in CRC patients. Taken together, stratifying patients according to HER2 expression can help in the diagnosis and prognosis of CRC patients from North African origin.

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HER2过表达被认为是北非晚期结直肠癌患者的诊断和预后生物标志物。

目的:结直肠癌(CRC)是世界上主要的癌症之一。尽管其死亡率和病理生理学在美国和欧洲国家有很好的记录，但很少在北非人群中进行研究。近期研究表明HER2过表达与食管癌和胃癌有关。本研究的目的是评估利比亚结直肠癌患者HER2蛋白和mRNA的表达及其与肿瘤发病机制的相关性。方法:从原发性结直肠癌患者共收集17个FFPE组织块。免疫组化法检测HER2蛋白表达，qRT-PCR法检测mRNA表达。另外165例患者进行了HER2过表达在直肠腺癌中的生存分析。结果:在结直肠癌队列中，腺癌更为常见，占88.2%，粘液腺癌占11.8%。约47%的病例HER2阳性(评分≥2+)，约50%的II级腺癌患者HER2阳性。此外，57.4%的ii级腺癌患者行了右半结肠切除术。HER2 mRNA的表达与肿瘤分级有显著相关(p = 0.03)。此外，在直肠腺癌中，HER2高表达的患者总体生存率较低。结论:据我们所知，这是首次研究HER2过表达与北非人群中更具侵袭性的结直肠癌相关。我们的研究表明HER2过表达与右结肠手术有关。此外，mRNA和蛋白表达的相关性可以保证在结直肠癌患者中实施HER2阳性的全国性筛查计划。综上所述，根据HER2表达对患者进行分层有助于北非结直肠癌患者的诊断和预后。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.