Gene expression analysis in NSAID-induced rat small intestinal disease model with the intervention of berberine by the liquid chip technology.

IF 2.7 4区 医学 Q2 GENETICS & HEREDITY
Guanqun Chao, Qianqian Wang, Fangxu Ye, Shuo Zhang
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引用次数: 0

Abstract

Objective: Investigate the effect and mechanism of berberine on the small intestinal mucosa of non-steroidal anti-inflammatory drugs (NSAIDs) related small intestinal injury.

Materials and methods: Twenty-four SD rats were randomly divided into control group, model group and intervention group. The model group and intervention group were treated with diclofenac (7.5 mg/kg·d, 2/d), a total of 4 days tube feeding, and the intervention group was treated with 50 mg/kg·d intragastric administration of berberine after 2 days. The control group was treated with 7.5 mg/kg·d, 2/d 0.9% saline tube feeding. Then we screened differential expression of colonic mucosal gene by the liquid chip technology.

Results: Compared with the control group, macroscopic and histology score of the model group increased significantly (P < 0.05), HTR4, HTR1a, F2RL3, CALCA, NPY, CRHR2, IL1b, P2RX3, TPH1, HMOX1, TRPV1, VIP, F2RL1, SLC6A4, TFF2, AQP8 content were significantly increased (P < 0.05), NOS1 content decreased significantly (P < 0.05); Compared with the model group, macroscopic and histology score of the intervention group improved significantly (P < 0.05), and HTR4, F2RL3, NPY, CRHR2, IL1b, VIP, AQP8 content were significantly lower (P < 0.05), NOS1 content increased significantly (P < 0.05).

Conclusion: Berberine has a protective effect on NSAID-associated small intestinal injury, the mechanism may be that berberine decreases the expression of intestinal mucosa HTR4, F2RL3, NPY, CRHR2, IL1b, VIP, AQP8, and increases the expression of NOS1, that to reduce intestinal permeability and protect intestinal mucosal barrier.

Abstract Image

Abstract Image

用液体芯片技术分析小檗碱干预非甾体抗炎药诱导的大鼠小肠疾病模型的基因表达。
目的:探讨小檗碱对非甾体抗炎药(NSAIDs)相关小肠损伤小肠黏膜的影响及机制。材料与方法:将24只SD大鼠随机分为对照组、模型组和干预组。模型组和干预组大鼠分别给予双氯芬酸(7.5 mg/kg·d, 2 mg/ d)灌胃,共4 d,干预组大鼠于2 d后给予小檗碱50 mg/kg·d灌胃。对照组给予7.5 mg/kg·d, 0.9%生理盐水管饲2次/d。然后利用液体芯片技术筛选结肠粘膜基因的差异表达。结果:与对照组相比,模型组大鼠宏观及组织学评分均显著升高(P)。结论:小檗碱对非甾体抗炎药相关性小肠损伤具有保护作用,其机制可能是通过降低肠道黏膜HTR4、F2RL3、NPY、CRHR2、IL1b、VIP、AQP8的表达,增加NOS1的表达,从而降低肠道通透性,保护肠黏膜屏障。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genes and Environment
Genes and Environment Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
4.00
自引率
0.00%
发文量
24
审稿时长
27 weeks
期刊介绍: Genes and Environment is an open access, peer-reviewed journal that aims to accelerate communications among global scientists working in the field of genes and environment. The journal publishes articles across a broad range of topics including environmental mutagenesis and carcinogenesis, environmental genomics and epigenetics, molecular epidemiology, genetic toxicology and regulatory sciences. Topics published in the journal include, but are not limited to, mutagenesis and anti-mutagenesis in bacteria; genotoxicity in mammalian somatic cells; genotoxicity in germ cells; replication and repair; DNA damage; metabolic activation and inactivation; water and air pollution; ROS, NO and photoactivation; pharmaceuticals and anticancer agents; radiation; endocrine disrupters; indirect mutagenesis; threshold; new techniques for environmental mutagenesis studies; DNA methylation (enzymatic); structure activity relationship; chemoprevention of cancer; regulatory science. Genetic toxicology including risk evaluation for human health, validation studies on testing methods and subjects of guidelines for regulation of chemicals are also within its scope.
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