Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats.

IF 2.4 3区 医学 Q2 ANDROLOGY
Jasmina Sretenovic, Jovana Joksimovic Jovic, Ivan Srejovic, Vladimir Zivkovic, Katarina Mihajlovic, Milica Labudovic-Borovic, Svetlana Trifunovic, Verica Milosevic, Dejan Lazic, Sergey Bolevich, Vladimir Jakovljevic, Zoran Milosavljevic
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引用次数: 5

Abstract

Background: During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH).

Results: Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control.

Conclusions: Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction.

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癸酸诺龙和游泳治疗的成年雄性大鼠睾丸形态计量学分析和氧化还原状态。
背景:在过去的几十年里,滥用合成代谢雄激素类固醇(AASs)在专业和休闲运动员中变得很流行。滥用AASs会导致性激素水平下降,但是关于单独游泳或与AASs一起游泳对睾丸组织的影响,现有文献提供的数据非常少。本研究的目的是研究为期四周的癸酸诺龙和游泳训练单独或联合对睾丸组织形态计量参数、雄激素受体(AR)和氧化还原状态的影响。选取10周龄Wistar白化雄性大鼠,分为4组:对照组(T-N-)、诺龙组(T-N+)、游泳训练组(T+N-)和诺龙游泳训练组(T+N+)。诺龙(N+)组大鼠给予癸酸诺龙20 mg/kg b.w.w,每周1次。训练(T+)组大鼠游泳1 h/天,每周5天。对分离的睾丸进行测量,左侧睾丸常规处理进行组织学分析,右侧睾丸均质化并准备用于分析以下氧化应激生物标志物:脂质过氧化指数(TBARS)、亚硝酸盐、过氧化氢酶、超氧化物歧化酶(SOD)和还原性谷胱甘肽(GSH)。结果:与对照组相比,T+N+组的精管直径和截面积分别减少10%和21%,T+N+组的精管直径和截面积分别减少15%和41%。各试验组睾丸间质均减少。与对照组相比,T+N+组AR免疫反应性降低22%,T+N+组AR免疫反应性降低9%。T+N-组和T+N+组TBARS水平升高。T+N+组亚硝酸盐含量降低。各试验组过氧化氢酶活性均升高。与对照组相比,单独游泳或与诺龙联合游泳可降低GSH水平。与对照组相比,T-N+和T+N+组SOD活性降低。结论:诺龙单独或联合游泳可降低睾丸组织形态测量参数和AR含量。氧化还原状态的变化表明生殖功能障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Basic and Clinical Andrology
Basic and Clinical Andrology Medicine-Urology
CiteScore
3.50
自引率
0.00%
发文量
21
审稿时长
22 weeks
期刊介绍: Basic and Clinical Andrology is an open access journal in the domain of andrology covering all aspects of male reproductive and sexual health in both human and animal models. The journal aims to bring to light the various clinical advancements and research developments in andrology from the international community.
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