Exercise-Mediated Alteration of miR-192-5p Is Associated with Cognitive Improvement in Alzheimer's Disease.

IF 2.2 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Neuroimmunomodulation Pub Date : 2022-01-01 Epub Date: 2021-07-13 DOI:10.1159/000516928
Zhaomei Qin, Xingjun Han, Jing Ran, Shanshan Guo, Lina Lv
{"title":"Exercise-Mediated Alteration of miR-192-5p Is Associated with Cognitive Improvement in Alzheimer's Disease.","authors":"Zhaomei Qin,&nbsp;Xingjun Han,&nbsp;Jing Ran,&nbsp;Shanshan Guo,&nbsp;Lina Lv","doi":"10.1159/000516928","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Physical exercise is an important component of managing Alzheimer's disease (AD). miRNAs can be modulated by exercise intervention.</p><p><strong>Objective: </strong>The study explored the involvement and potential mechanism of miR-192-5p in the protective effect of physical exercise on AD.</p><p><strong>Methods: </strong>Ninety AD patients were enrolled, in which 45 cases accepted cycling training for continuous 3 months. The expression changes of miR-192-5p before and after exercise were analyzed by reverse transcription-quantitative PCR. 8-month-old APP/PS1 double Tg mice were used as the AD animal model. Mice in the voluntary exercise (VE) group received VE for 4 weeks. Morris water maze (MWM) test was used to evaluate the learning and memory function. Enzyme-linked immunosorbent assay was used to calculate the level of IL-1β, IL-6, and TNF-α.</p><p><strong>Results: </strong>AD patients showed elevated MMSE scores, decreased ADAS-cog and NPI-Q scores after 3 months of exercise. miR-192-5p was downregulated in the serum of AD patients and correlated with the levels of MMSE score, ADAS-cog, and NPI-Q score. A positive association was detected between serum miR-192-5p with TNF-α, IL-6, and IL-1β levels. MiR-192-5p is downregulated in the hippocampus tissues of mice after VE. Overexpression of miR-192-5p reversed the neuroprotective effect of exercise on AD in mice and promoted the inflammatory response of AD mice.</p><p><strong>Conclusion: </strong>MiR-192-5p can be modulated by the exercise intervention and involved in the protective effect of exercise on AD.</p>","PeriodicalId":19133,"journal":{"name":"Neuroimmunomodulation","volume":"29 1","pages":"36-43"},"PeriodicalIF":2.2000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuroimmunomodulation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000516928","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/7/13 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 6

Abstract

Introduction: Physical exercise is an important component of managing Alzheimer's disease (AD). miRNAs can be modulated by exercise intervention.

Objective: The study explored the involvement and potential mechanism of miR-192-5p in the protective effect of physical exercise on AD.

Methods: Ninety AD patients were enrolled, in which 45 cases accepted cycling training for continuous 3 months. The expression changes of miR-192-5p before and after exercise were analyzed by reverse transcription-quantitative PCR. 8-month-old APP/PS1 double Tg mice were used as the AD animal model. Mice in the voluntary exercise (VE) group received VE for 4 weeks. Morris water maze (MWM) test was used to evaluate the learning and memory function. Enzyme-linked immunosorbent assay was used to calculate the level of IL-1β, IL-6, and TNF-α.

Results: AD patients showed elevated MMSE scores, decreased ADAS-cog and NPI-Q scores after 3 months of exercise. miR-192-5p was downregulated in the serum of AD patients and correlated with the levels of MMSE score, ADAS-cog, and NPI-Q score. A positive association was detected between serum miR-192-5p with TNF-α, IL-6, and IL-1β levels. MiR-192-5p is downregulated in the hippocampus tissues of mice after VE. Overexpression of miR-192-5p reversed the neuroprotective effect of exercise on AD in mice and promoted the inflammatory response of AD mice.

Conclusion: MiR-192-5p can be modulated by the exercise intervention and involved in the protective effect of exercise on AD.

运动介导的miR-192-5p的改变与阿尔茨海默病的认知改善有关
体育锻炼是控制阿尔茨海默病(AD)的重要组成部分。mirna可以通过运动干预来调节。目的:探讨miR-192-5p在体育锻炼对AD的保护作用中的作用及其潜在机制。方法:入选90例AD患者,其中45例接受连续3个月的自行车训练。采用逆转录-定量PCR分析运动前后miR-192-5p的表达变化。采用8月龄APP/PS1双Tg小鼠作为AD动物模型。自愿运动(VE)组小鼠连续4周接受VE治疗。Morris水迷宫(Morris water maze, MWM)测试大鼠的学习记忆功能。采用酶联免疫吸附法计算IL-1β、IL-6、TNF-α水平。结果:运动3个月后,AD患者MMSE评分升高,ADAS-cog和NPI-Q评分降低。AD患者血清中miR-192-5p水平下调,与MMSE评分、ADAS-cog、NPI-Q评分水平相关。血清miR-192-5p与TNF-α、IL-6和IL-1β水平呈正相关。VE后小鼠海马组织中MiR-192-5p表达下调。miR-192-5p的过表达逆转了运动对小鼠AD的神经保护作用,促进了AD小鼠的炎症反应。结论:MiR-192-5p可通过运动干预调节,参与运动对AD的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Neuroimmunomodulation
Neuroimmunomodulation 医学-免疫学
CiteScore
3.60
自引率
4.20%
发文量
35
审稿时长
>12 weeks
期刊介绍: The rapidly expanding area of research known as neuroimmunomodulation explores the way in which the nervous system interacts with the immune system via neural, hormonal, and paracrine actions. Encompassing both basic and clinical research, ''Neuroimmunomodulation'' reports on all aspects of these interactions. Basic investigations consider all neural and humoral networks from molecular genetics through cell regulation to integrative systems of the body. The journal also aims to clarify the basic mechanisms involved in the pathogenesis of the CNS pathology in AIDS patients and in various neurodegenerative diseases. Although primarily devoted to research articles, timely reviews are published on a regular basis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信