Connexins in the development and physiology of stem cells.

IF 3.6 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Tissue Barriers Pub Date : 2021-10-02 Epub Date: 2021-07-06 DOI:10.1080/21688370.2021.1949242

Anaclet Ngezahayo, Frederike A Ruhe

引用次数: 4

Abstract

Connexins (Cxs) form gap junction (GJ) channels linking vertebrate cells. During embryogenesis, Cxs are expressed as early as the 4-8 cell stage. As cells differentiate into pluripotent stem cells (PSCs) and during gastrulation, the Cx expression pattern is adapted. Knockdown of Cx43 and Cx45 does not interfere with embryogenic development until the blastula stage, questioning the role of Cxs in PSC physiology and development. Studies in cultivated and induced PSCs (iPSCs) showed that Cx43 is essential for the maintenance of self-renewal and the expression of pluripotency markers. It was found that the role of Cxs in PSCs is more related to regulation of transcription or cell-cell adherence than to formation of GJ channels. Furthermore, a crucial role of Cxs for the self-renewal and differentiation was shown in cultivated adult mesenchymal stem cells. This review aims to highlight aspects that link Cxs to the function and physiology of stem cell development.

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干细胞发育和生理中的连接蛋白。

连接蛋白(Cxs)形成连接脊椎动物细胞的间隙连接(GJ)通道。在胚胎发生过程中，早在4-8细胞阶段就表达了xs。当细胞分化为多能干细胞(PSCs)和原肠胚形成时，Cx的表达模式发生了变化。在囊胚期之前，Cx43和Cx45的敲低并不会干扰胚胎发育，这就质疑了Cxs在PSC生理和发育中的作用。对培养和诱导的PSCs (iPSCs)的研究表明，Cx43对于维持自我更新和多能性标志物的表达至关重要。研究发现，与GJ通道的形成相比，xs在PSCs中的作用更多的是与转录或细胞-细胞粘附的调节有关。此外，在培养的成体间充质干细胞中，xs在自我更新和分化中起着至关重要的作用。本文综述了Cxs与干细胞发育的功能和生理之间的联系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue Barriers MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

6.60

自引率

6.50%

发文量

期刊介绍： Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.