Isolation and characterization of chemical constituents from Chaerophyllum bulbosum roots and their enzyme inhibitory and antioxidant effects.

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences Pub Date : 2021-07-06 Print Date: 2022-01-27 DOI:10.1515/znc-2021-0119

Gülsen Tel-Çayan, Ebru Deveci, Zeynep Molo, Mehmet Emin Duru, Mehmet Öztürk

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引用次数: 1

Abstract

Isolation and bioactive effects of the roots of Chaerophyllum bulbosum L. were firstly investigated herein. Enzyme (acetylcholinesterase, butyrylcholinesterase, urease, α-amylase, α-glucosidase, and tyrosinase) inhibitory effects of C. bulbosum root extracts were tested. Three known compounds, n-heptadecanyl eicosanoate (1), stigmasterol (2), and β-sitosterol-3-O-β-d-glucopyranoside (3) were isolated from C. bulbosum. Antioxidant and enzyme inhibitory effects of isolated compounds were investigated. The hexane extract (IC₅₀: 349.58 ± 0.06 μg/mL) displayed a higher α-glucosidase inhibitory effect than the standard (IC₅₀: 378.66 ± 0.14 μg/mL). The best inhibitory effect was found in compound 2 on AChE (46.40 ± 0.31%), BChE (56.41 ± 0.54%), and urease (92.47 ± 0.11%); compound 1 on α-amylase (22.27 ± 0.61%); and compound 3 on α-glucosidase (12.43 ± 0.25%) and tyrosinase (19.00 ± 0.16%). All isolated compounds showed moderate antioxidant effects in all assays. This study contributes to the therapeutic uses of Chaerophyllum roots and emphasizes the value of C. bulbosum species for the development of novel therapeutic agents.

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毛竹根化学成分的分离鉴定及其酶抑制和抗氧化作用。

本文首次研究了毛胚(Chaerophyllum bulbosum L.)根的分离及其生物活性。考察了根提取物对乙酰胆碱酯酶、丁酰胆碱酯酶、脲酶、α-淀粉酶、α-葡萄糖苷酶和酪氨酸酶的抑制作用。从球芽草中分离得到3个已知化合物，n-庚烷基二十烷酸酯(1)、豆甾醇(2)和β-谷甾醇-3- 0 -β-d-葡萄糖吡喃苷(3)。研究了分离化合物的抗氧化和酶抑制作用。正己烷提取物(IC50: 349.58±0.06 μg/mL)对α-葡萄糖苷酶的抑制作用明显高于对照品(IC50: 378.66±0.14 μg/mL)。化合物2对AChE(46.40±0.31%)、BChE(56.41±0.54%)和脲酶(92.47±0.11%)的抑制效果最好;化合物1作用于α-淀粉酶(22.27±0.61%);化合物3作用于α-葡萄糖苷酶(12.43±0.25%)和酪氨酸酶(19.00±0.16%)。所有分离的化合物在所有实验中都显示出中等的抗氧化作用。本研究有助于研究毛藻根的治疗用途，并强调了毛藻在新型治疗剂开发中的价值。

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来源期刊

Zeitschrift Fur Naturforschung Section C-A Journal of Biosciences 生物-生化与分子生物学

CiteScore

4.10

自引率

5.00%

发文量

期刊介绍： A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) is an international scientific journal and a community resource for the emerging field of natural and natural-like products. The journal publishes original research on the isolation (including structure elucidation), bio-chemical synthesis and bioactivities of natural products, their biochemistry, pharmacology, biotechnology, and their biological activity and innovative developed computational methods for predicting the structure and/or function of natural products. A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) welcomes research papers in fields on the chemistry-biology boundary which address scientific ideas and approaches to generate and understand natural compounds on a molecular level and/or use them to stimulate and manipulate biological processes.