Effects of galectin-1 on immunomodulatory properties of human monocyte-derived dendritic cells.

IF 1.8 4区生物学 Q4 CELL BIOLOGY

Growth factors Pub Date : 2020-12-01 Epub Date: 2021-07-05 DOI:10.1080/08977194.2021.1947267

Tanja Džopalić, Miloš Kostić, Milena Kostić, Goran Marjanović, Jelena Guzina, Vladimir Jurišić, Biljana Božić Nedeljković

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引用次数: 5

Abstract

Our study aimed to evaluate the effects of Gal-1 in dose depending manner on maturation and immunomodulatory properties of monocyte-derived (Mo) DCs in-vitro. The effects were analyzed by monitoring their phenotypic characteristics, cytokine profile, and the ability to direct the immune response in the co-culture with allogeneic CD4⁺T cells. Gal-1 reduced the expression of CD80 and CD86 molecules on MoDCs compared to untreated MoDCs. Gal-1 at concentrations of 1 and 6 μg/mL significantly reduced IL-12 production, while the concentration of 3 μg/mL led to its significant increase. Gal-1 in all concentrations induced a significant increase in the production of IL-10. Treatment of MoDCs with 3 and 6 μg/mL of Gal-1 stimulated the production of IL-2 and IFN-γ in the co-culture with CD4⁺T lymphocytes. This study demonstrated a dual immunomodulatory effect of Gal-1 on MoDCs in terms of immune stimulation and immune suppression, depending on the applied concentration.

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半乳糖凝集素-1对人单核细胞源性树突状细胞免疫调节特性的影响。

我们的研究旨在评估Gal-1剂量依赖性对体外单核细胞源性dc成熟和免疫调节特性的影响。通过监测它们的表型特征、细胞因子谱和在与异体CD4+T细胞共培养中指导免疫反应的能力来分析这些影响。与未处理的MoDCs相比，Gal-1降低了MoDCs上CD80和CD86分子的表达。1和6 μg/mL浓度的Gal-1显著降低IL-12的生成，3 μg/mL浓度的Gal-1显著增加IL-12的生成。所有浓度的Gal-1均诱导IL-10的产生显著增加。用3和6 μg/mL Gal-1处理MoDCs可刺激CD4+T淋巴细胞共培养IL-2和IFN-γ的产生。本研究表明，Gal-1对MoDCs具有免疫刺激和免疫抑制的双重免疫调节作用，其作用取决于应用浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Growth factors 生物-内分泌学与代谢

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications