Low-Intensity Pulsed Ultrasound Enhances Neurotrophic Factors and Alleviates Neuroinflammation in a Rat Model of Parkinson's Disease.

IF 2.9 2区医学 Q2 NEUROSCIENCES

Cerebral cortex Pub Date : 2021-11-23 DOI:10.1093/cercor/bhab201

Chen-Yu Sung, Pai-Kai Chiang, Che-Wen Tsai, Feng-Yi Yang

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引用次数: 14

Abstract

Low-intensity pulsed ultrasound (LIPUS) has also been reported to improve behavioral functions in Parkinson's disease (PD) animal models; however, the effect of LIPUS stimulation on the neurotrophic factors and neuroinflammation has not yet been addressed. PD rat model was built by injection of 6-hydroxydopamine (6-OHDA) in 2 sites in the right striatum. The levels of neurotrophic factors and lipocalin-2 (LCN2)-induced neuroinflammation were quantified using a western blot. Rotational test and cylinder test were conducted biweekly for 8 weeks. When the 6-OHDA + LIPUS and 6-OHDA groups were compared, the locomotor function of the 6-OHDA + LIPUS rats was significantly improved. After LIPUS stimulation, the tyrosine hydroxylase staining density was significantly increased in the striatum and substantia nigra pars compacta (SNpc) of lesioned rats. Unilateral LIPUS stimulation did not increase brain-derived neurotrophic factor in the striatum and SNpc of lesioned rats. In contrast, unilateral LIPUS stimulation increased glial cell line-derived neurotrophic factor (GDNF) protein 1.98-fold unilaterally in the SNpc. Additionally, LCN2-induced neuroinflammation can be attenuated following LIPUS stimulation. Our data indicated that LIPUS stimulation may be a potential therapeutic tool against PD via enhancement of GDNF level and inhibition of inflammatory responses in the SNpc of the brain.

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低强度脉冲超声增强帕金森病大鼠模型的神经营养因子并减轻神经炎症

据报道，低强度脉冲超声(LIPUS)也能改善帕金森病(PD)动物模型的行为功能;然而，LIPUS刺激对神经营养因子和神经炎症的影响尚未得到解决。在右侧纹状体2个部位注射6-羟基多巴胺(6-OHDA)，建立PD大鼠模型。神经营养因子和脂载素-2 (LCN2)诱导的神经炎症水平采用western blot定量。旋转试验和圆筒试验每两周进行一次，共8周。6-OHDA + LIPUS组与6-OHDA组比较，6-OHDA + LIPUS组大鼠运动功能明显改善。LIPUS刺激后，损伤大鼠纹状体和致密黑质(SNpc)的酪氨酸羟化酶染色密度显著增加。单侧LIPUS刺激未增加损伤大鼠纹状体脑源性神经营养因子和SNpc。相反，单侧LIPUS刺激使SNpc中神经胶质细胞系来源的神经营养因子(GDNF)蛋白单侧增加1.98倍。此外，lcn2诱导的神经炎症可以在LIPUS刺激后减轻。我们的数据表明，LIPUS刺激可能是一种潜在的治疗工具，通过增强GDNF水平和抑制大脑SNpc的炎症反应来治疗PD。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cerebral cortex 医学-神经科学

CiteScore

6.30

自引率

8.10%

发文量

510

审稿时长

2 months

期刊介绍： Cerebral Cortex publishes papers on the development, organization, plasticity, and function of the cerebral cortex, including the hippocampus. Studies with clear relevance to the cerebral cortex, such as the thalamocortical relationship or cortico-subcortical interactions, are also included. The journal is multidisciplinary and covers the large variety of modern neurobiological and neuropsychological techniques, including anatomy, biochemistry, molecular neurobiology, electrophysiology, behavior, artificial intelligence, and theoretical modeling. In addition to research articles, special features such as brief reviews, book reviews, and commentaries are included.