Identification of Two Similar Novel HIV-1 Recombinant Forms (CRF01_AE/CRF07_BC) among Intravenous Drug Users in Guangxi, China.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2022-01-01 Epub Date: 2021-06-29 DOI:10.1159/000517052

Fei Zhang, Yao Yang, Bingyu Liang, Yuan Yang, Qiuyu Wei, Peijiang Pan, Li Ye, Hao Liang

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引用次数: 1

Abstract

New kinds of HIV-1 circulating recombinant forms (CRFs) and unique recombinant forms (URFs) earn a great prevalence in China nowadays. In this study, we identified 2 similar URFs (2016GXNNIDU037 and 2019QZLSIDU253) both isolated from intravenous drug users (IDUs) in Guangxi, China. Phylogenetic analysis of the near full-length genome (NFLG) revealed 2 URFs both clustered with CRF01_AE but setting up a monophyletic branch, supporting a high bootstrap value. Bootscan analysis and subregional recombinant analysis found that the NFLG of 2016GXNNIDU037 and 2019QZLSIDU253 were both composed of CRF01_AE and CRF07_BC, with 3 CRF07_BC mosaic segments inserted into CRF01_AE backbones. The CRF01_AE segments of the 2 URFs clustered with a previously reported cluster 2 lineage of CRF01_AE. The 5 recombinant breakpoints of the 2 URFs were quite similar. Distinct from CRF01_AE/CRF07_BC URFs reported before, 2016GXNNIDU037 and 2019QZLSIDU253 are new evidence of a high genetic variety of HIV-1 in Guangxi, which may pose new challenges to HIV-1 prevention and molecular epidemiological surveillance in China.

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广西静脉注射吸毒者中两种相似的新型HIV-1重组基因CRF01_AE/CRF07_BC的鉴定

目前，新型HIV-1循环重组形式(CRFs)和独特重组形式(URFs)在中国广泛流行。在这项研究中，我们从中国广西的静脉吸毒者(IDUs)中分离出2个相似的urf (2016GXNNIDU037和2019QZLSIDU253)。近全长基因组(NFLG)的系统发育分析显示，2个urf均与CRF01_AE聚集，但建立了一个单系分支，支持较高的自举值。Bootscan分析和分区域重组分析发现，2016gxnidu037和2019QZLSIDU253的NFLG均由CRF01_AE和CRF07_BC组成，其中3个CRF07_BC镶嵌片段插入CRF01_AE主干。2个urf的CRF01_AE片段与先前报道的CRF01_AE的集群2谱系聚集在一起。2个urf的5个重组断点非常相似。与之前报道的CRF01_AE/CRF07_BC URFs不同，2016GXNNIDU037和2019QZLSIDU253是广西HIV-1高遗传多样性的新证据，可能对中国HIV-1预防和分子流行病学监测提出新的挑战。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567