Identification of Two Similar Novel HIV-1 Recombinant Forms (CRF01_AE/CRF07_BC) among Intravenous Drug Users in Guangxi, China.

IF 3.2 4区医学 Q3 VIROLOGY

Intervirology Pub Date : 2022-01-01 Epub Date: 2021-06-29 DOI:10.1159/000517052

Fei Zhang, Yao Yang, Bingyu Liang, Yuan Yang, Qiuyu Wei, Peijiang Pan, Li Ye, Hao Liang

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引用次数: 1

Abstract

New kinds of HIV-1 circulating recombinant forms (CRFs) and unique recombinant forms (URFs) earn a great prevalence in China nowadays. In this study, we identified 2 similar URFs (2016GXNNIDU037 and 2019QZLSIDU253) both isolated from intravenous drug users (IDUs) in Guangxi, China. Phylogenetic analysis of the near full-length genome (NFLG) revealed 2 URFs both clustered with CRF01_AE but setting up a monophyletic branch, supporting a high bootstrap value. Bootscan analysis and subregional recombinant analysis found that the NFLG of 2016GXNNIDU037 and 2019QZLSIDU253 were both composed of CRF01_AE and CRF07_BC, with 3 CRF07_BC mosaic segments inserted into CRF01_AE backbones. The CRF01_AE segments of the 2 URFs clustered with a previously reported cluster 2 lineage of CRF01_AE. The 5 recombinant breakpoints of the 2 URFs were quite similar. Distinct from CRF01_AE/CRF07_BC URFs reported before, 2016GXNNIDU037 and 2019QZLSIDU253 are new evidence of a high genetic variety of HIV-1 in Guangxi, which may pose new challenges to HIV-1 prevention and molecular epidemiological surveillance in China.

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广西静脉注射吸毒者中两种相似的新型HIV-1重组基因CRF01_AE/CRF07_BC的鉴定

目前，新型HIV-1循环重组形式(CRFs)和独特重组形式(URFs)在中国广泛流行。在这项研究中，我们从中国广西的静脉吸毒者(IDUs)中分离出2个相似的urf (2016GXNNIDU037和2019QZLSIDU253)。近全长基因组(NFLG)的系统发育分析显示，2个urf均与CRF01_AE聚集，但建立了一个单系分支，支持较高的自举值。Bootscan分析和分区域重组分析发现，2016gxnidu037和2019QZLSIDU253的NFLG均由CRF01_AE和CRF07_BC组成，其中3个CRF07_BC镶嵌片段插入CRF01_AE主干。2个urf的CRF01_AE片段与先前报道的CRF01_AE的集群2谱系聚集在一起。2个urf的5个重组断点非常相似。与之前报道的CRF01_AE/CRF07_BC URFs不同，2016GXNNIDU037和2019QZLSIDU253是广西HIV-1高遗传多样性的新证据，可能对中国HIV-1预防和分子流行病学监测提出新的挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Intervirology 医学-病毒学

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.