Candida auris: Diagnostic Challenges and Emerging Opportunities for the Clinical Microbiology Laboratory.

IF 2.3 Q3 INFECTIOUS DISEASES

Current Fungal Infection Reports Pub Date : 2021-01-01 Epub Date: 2021-06-23 DOI:10.1007/s12281-021-00420-y

C Keighley, K Garnham, S A J Harch, M Robertson, K Chaw, J C Teng, S C-A Chen

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引用次数: 18

Abstract

Purpose of review: This review summarises the epidemiology of Candida auris infection and describes contemporary and emerging diagnostic methods for detection and identification of C. auris.

Recent findings: A fifth C. auris clade has been described. Diagnostic accuracy has improved with development of selective/differential media for C. auris. Advances in spectral databases of matrix-associated laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) systems have reduced misidentification. Direct detection of C. auris in clinical specimens using real time PCR is increasingly used, as is whole genome sequencing (WGS) to track nosocomial spread and to study phylogenetic relationships and drug resistance.

Summary: C. auris is an important transmissible, nosocomial pathogen. The microbiological laboratory diagnostic capacity has extended beyond culture-based methods to include PCR and WGS. Microbiological techniques on the horizon include the use of MALDI-TOF MS for early echinocandin antifungal susceptibility testing (AST) and expansion of the versatile and information-rich WGS methods for outbreak investigation.

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耳念珠菌:临床微生物实验室的诊断挑战和新机遇。

综述目的:本文综述了耳念珠菌感染的流行病学，并介绍了检测和鉴定耳念珠菌的当代和新兴诊断方法。最近的发现:已经描述了第五个C. auris分支。随着auris选择性/鉴别培养基的发展，诊断准确性得到了提高。基体相关激光解吸电离飞行时间质谱(MALDI-TOF MS)系统光谱数据库的进步减少了错误识别。利用实时PCR技术直接检测临床标本中的金黄色葡萄球菌，以及利用全基因组测序(WGS)来追踪院内传播、研究系统发育关系和耐药性也越来越多地得到应用。摘要:耳念珠菌是一种重要的传染性医院病原菌。微生物实验室诊断能力已经超越了基于培养的方法，包括PCR和WGS。即将出现的微生物学技术包括使用MALDI-TOF质谱进行早期刺白菌素抗真菌药敏试验(AST)，以及扩展多功能和信息丰富的WGS方法用于暴发调查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Fungal Infection Reports INFECTIOUS DISEASES-

CiteScore

3.10

自引率

7.10%

发文量

期刊介绍： This journal intends to provide clear, insightful, balanced contributions that review the most important, recently published clinical findings related to the diagnosis, treatment, management, and prevention of fungal infections. We accomplish this aim by appointing international authorities to serve as Section Editors in key subject areas, such as advances in diagnosis, current and emerging management approaches, and genomics and pathogenesis. Section Editors, in turn, select topics for which leading experts contribute comprehensive review articles that emphasize new developments and recently published papers of major importance, highlighted by annotated reference lists. Commentaries from well-known figures in the field are also provided, and an international Editorial Board reviews the annual table of contents, suggests articles of special interest to their country/region, and ensures that topics are current and include emerging research.