Integrated proteomics and metabolomics analysis of transgenic and gene-stacked maize line seeds.

IF 4.5 2区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2021-01-02 DOI:10.1080/21645698.2021.1934351

Weixiao Liu, Haiming Zhao, Chaohua Miao, Wujun Jin

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引用次数: 9

Abstract

Unintended effects of genetically modified (GM) crops may pose safety issues. Omics techniques provide researchers with useful tools to assess such unintended effects. Proteomics and metabolomics analyses were performed for three GM maize varieties, 2A-7, CC-2, and 2A-7×CC-2 stacked transgenic maize, and the corresponding non-GM parent Zheng58.Proteomics revealed 120, 271 and 135 maize differentially expressed proteins (DEPs) in the 2A-7/Zheng58, CC-2/Zheng58 and 2A-7×CC-2/Zheng58 comparisons, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most DEPs participated in metabolic pathways and the biosynthesis of secondary metabolite. Metabolomics revealed 179, 135 and 131 differentially accumulated metabolites (DAMs) in the 2A-7/Zheng58, CC-2/Zheng58 and 2A-7×CC-2/Zheng58 comparisons, respectively. Based on KEGG enrichment analysis, most DAMs are involved in the biosynthesis of secondary metabolite and metabolic pathways. According to integrated proteomics and metabolomics analysis, the introduction of exogenous EPSPS did not affect the expression levels of six other enzymes or the abundance of seven metabolites involved in the shikimic acid pathway in CC-2 and 2A-7×CC-2 seeds. Six co-DEPs annotated by integrated proteomics and metabolomics pathway analysis were further analyzed by qRT-PCR.This study successfully employed integrated proteomic and metabolomic technology to assess unintended changes in maize varieties. The results suggest that GM and gene stacking do not cause significantly unintended effects.

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转基因和基因堆叠玉米品系种子的综合蛋白质组学和代谢组学分析。

转基因作物的意外影响可能会带来安全问题。组学技术为研究人员提供了有用的工具来评估这些意想不到的影响。对2A-7、CC-2和2A-7×CC-2三个转基因玉米品种及其对应的非转基因亲本郑58进行了蛋白质组学和代谢组学分析。蛋白质组学分析显示，2A-7/Zheng58、CC-2/Zheng58和2A-7×CC-2/Zheng58分别含有120、271和135个玉米差异表达蛋白(DEPs)。京都基因与基因组百科(KEGG)途径富集分析表明，大多数DEPs参与了代谢途径和次生代谢物的生物合成。代谢组学分析显示，2A-7/Zheng58、CC-2/Zheng58和2A-7×CC-2/Zheng58分别有179、135和131个差异积累代谢物(DAMs)。基于KEGG富集分析，大多数dam参与了次生代谢物的生物合成和代谢途径。结合蛋白质组学和代谢组学分析，外源EPSPS的引入并未影响CC-2和2A-7×CC-2种子中莽草酸通路中其他6种酶的表达水平和7种代谢物的丰度。通过综合蛋白质组学和代谢组学通路分析对6个co-DEPs进行进一步的qRT-PCR分析。本研究成功地采用综合蛋白质组学和代谢组学技术来评估玉米品种的非预期变化。结果表明，转基因和基因堆叠不会造成明显的意外影响。

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来源期刊

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

8.10

自引率

10.30%

发文量

期刊介绍： GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers. GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer. Topics covered include, but are not limited to: • Production and analysis of transgenic crops • Gene insertion studies • Gene silencing • Factors affecting gene expression • Post-translational analysis • Molecular farming • Field trial analysis • Commercialization of modified crops • Safety and regulatory affairs BIOLOGICAL SCIENCE AND TECHNOLOGY • Biofuels • Data from field trials • Development of transformation technology • Elimination of pollutants (Bioremediation) • Gene silencing mechanisms • Genome Editing • Herbicide resistance • Molecular farming • Pest resistance • Plant reproduction (e.g., male sterility, hybrid breeding, apomixis) • Plants with altered composition • Tolerance to abiotic stress • Transgenesis in agriculture • Biofortification and nutrients improvement • Genomic, proteomic and bioinformatics methods used for developing GM cops ECONOMIC, POLITICAL AND SOCIAL ISSUES • Commercialization • Consumer attitudes • International bodies • National and local government policies • Public perception, intellectual property, education, (bio)ethical issues • Regulation, environmental impact and containment • Socio-economic impact • Food safety and security • Risk assessments