Extracellular vesicles derived from lipoaspirate fluid promote fat graft survival.

IF 3.5 4区生物学 Q2 ENDOCRINOLOGY & METABOLISM

Adipocyte Pub Date : 2021-12-01 DOI:10.1080/21623945.2021.1932355

Fangfei Nie, Pengbing Ding, Chen Zhang, Zhenmin Zhao, Hongsen Bi

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引用次数: 4

Abstract

Extracellular vesicles (EVs) are specific subcellular vesicles released by cells under various environmental conditions. Tumescent liposuction is a commonly used procedure in plastic surgery practice. In the present study, we aimed to extract EVs derived from lipoaspirate fluid (LF-EVs) and characterize them using transmission electron microscopy, nanoparticle tracking analysis, and western blotting. The global profiles of proteins and microRNAs from LF-EVs were identified, strongly suggesting a potential regulatory function of LF-EVs. In addition, we investigated the effects and mechanisms of LF-EVs on fat graft survival. Cell functional tests showed that LF-EVs promoted the proliferation, migration, and tube structure formation of human umbilical vein endothelial cells. LF-EVs also promoted the adipogenic differentiation of adipose tissue-derived stem cells. The results of animal experiments showed that the average weights of fat grafts in the LF-EVs-treated group were significantly higher than those in the control group. Histologically, there was less fibrosis, fewer cysts, and increased fat tissue survival in the LF-EVs group. Further investigations of angiogenic and adipogenic factors revealed that LF-EVs also promoted angiogenesis and exerted a pro-adipogenic effect in vivo. Our findings will help to elucidate the functions of LF-EVs and provide a reference dataset for future translational studies.

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从吸脂液中提取的细胞外囊泡促进脂肪移植物存活。

细胞外囊泡(EVs)是细胞在各种环境条件下释放的特异性亚细胞囊泡。肿胀吸脂术是整形外科实践中常用的一种方法。在本研究中，我们旨在从抽脂液(lf - ev)中提取ev，并使用透射电子显微镜、纳米颗粒跟踪分析和免疫印迹法对其进行表征。研究人员鉴定了lf - ev的蛋白质和microrna的全局谱，强烈表明lf - ev具有潜在的调节功能。此外，我们还研究了lf - ev对脂肪移植存活的影响及其机制。细胞功能实验表明，lf - ev能促进人脐静脉内皮细胞的增殖、迁移和管状结构的形成。lf - ev还能促进脂肪组织源性干细胞的成脂分化。动物实验结果显示，lf - ev处理组脂肪移植体的平均重量明显高于对照组。组织学上，lf - ev组纤维化少，囊肿少，脂肪组织存活率提高。对血管生成因子和脂肪生成因子的进一步研究表明，lf - ev还能促进血管生成，并在体内发挥促脂肪作用。我们的发现将有助于阐明lf - ev的功能，并为未来的转化研究提供参考数据集。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Adipocyte Medicine-Histology

CiteScore

6.50

自引率

3.00%

发文量

审稿时长

32 weeks

期刊介绍： Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.