Growth and fatty acid composition of Acutodesmus obliquus under different light spectra and temperatures.

IF 1.8 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Lipids Pub Date : 2021-09-01 Epub Date: 2021-06-26 DOI:10.1002/lipd.12316
Mark Helamieh, Annkathrin Gebhardt, Marco Reich, Friedericke Kuhn, Martin Kerner, Klaus Kümmerer
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引用次数: 5

Abstract

The combined impact of temperature and light spectra on the fatty acid (FA) composition in microalgae has been sparsely investigated. The aim of this study was to investigate the interactions of light and temperature on the FA composition in Acutodesmus obliquus. For this purpose, A. obliquus was cultivated with different temperatures (20, 30, and 35°C), as well as broad light spectra (blue, green, and red light). Growth and FA composition were monitored daily. Microalgal FA were extracted, and a qualitative characterization was done by gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS). Compared to red light, green and blue light caused a higher percentage of the polyunsaturated fatty acids (PUFA) 16:4, 18:3, and 18:4, at all temperatures. The highest total percentage of these PUFA were observed at the lowest cultivation temperature and blue and green light. These data imply that a combination of lower temperatures and blue-green light (450-550 nm) positively influences the activity of specific FA-desaturases in A. obliquus. Additionally, a lower 16:1 trans/cis ratio was observed upon green and blue light treatment and lower cultivation temperatures. Remarkably, green light treatment resulted in a comparably high growth under all tested conditions. Therefore, a higher content of green light, compared to blue light might additionally lead to a higher biomass concentration. Microalgae cultivation with low temperatures and green light might therefore result in a suitable FA composition for the food industry and a comparably high biomass production.

不同光谱和温度下斜尖齿蚌的生长和脂肪酸组成。
温度和光谱对微藻中脂肪酸(FA)组成的综合影响研究很少。本研究的目的是探讨光和温度对斜针眼FA组成的相互作用。为此,我们在不同温度(20℃、30℃和35℃)和宽光谱(蓝光、绿光和红光)条件下培养斜叶草。每天监测生长和FA组成。提取微藻FA,采用气相色谱-电子冲击电离质谱(GC-EI/MS)对微藻FA进行定性分析。与红光相比,在所有温度下,绿光和蓝光引起的多不饱和脂肪酸(PUFA)的比例更高(16:4,18:3和18:4)。在最低的培养温度和蓝光、绿光条件下,这些多聚脂肪酸的总百分比最高。这些数据表明,较低温度和蓝绿光(450-550 nm)的组合对斜叶藻中特定fa -去饱和酶的活性有积极影响。此外,在绿光和蓝光处理和较低的培养温度下,观察到较低的16:1反式/顺式比。值得注意的是,在所有测试条件下,绿光处理导致了相对较高的生长。因此,与蓝光相比,较高的绿光含量可能会导致更高的生物量浓度。因此,在低温和绿光下培养微藻可能会产生适合食品工业的FA组成和相对较高的生物质产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Lipids
Lipids 生物-生化与分子生物学
CiteScore
4.20
自引率
5.30%
发文量
33
审稿时长
4-8 weeks
期刊介绍: Lipids is a journal of the American Oil Chemists'' Society (AOCS) that focuses on publishing high-quality peer-reviewed papers and invited reviews in the general area of lipid research, including chemistry, biochemistry, clinical nutrition, and metabolism. In addition, Lipids publishes papers establishing novel methods for addressing research questions in the field of lipid research.
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