Bovine Serum Albumin Conjugation in Superparamagnetic/Poly(methyl methacrylate) Nanoparticles as an Alternative for Magnetic Enzyme-Linked Immunosorbent Assays.

Journal of nanoscience and nanotechnology Pub Date : 2021-11-01 DOI:10.1166/jnn.2021.19458

Paulo Emilio Feuser, Camila Guindani, Jonathann Correa Possato, Jaqueline Pereira Guessi, Arthur Poester Cordeiro, Ricardo Andrez Machado-de-Ávila, Claudia Sayer, Pedro Henrique Hermes de Araújo

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引用次数: 1

Abstract

Nanomaterials, such as magnetic nanoparticles have attracted significant attention of medical area due to their capacity to improve the performance of immunoassays. Therefore the aim of this work was to study the bovine serum albumin (BSA) conjugation in superparamagnetic (MNPs)/poly(methyl methacrylate) (PMMA) nanoparticles with further characterization and application in enzyme-linked immunosorbent (ELISA) assay. The successful conjugation of BSA in MNPs- PMMA nanoparticles was confirmed by several techniques, including light scattering, zeta potential, transmission electron microscopy (TEM) and Lowry protein quantification assay. The superparamagnetic properties were confirmed by vibrating sample magnetometer. BSA conjugated MNPs-PMMA nanoparticles presented higher interactions with antibody than free BSA. The BSA + MNPs-PMMA nanoparticles (magnetic ELISA assay) reduced the time and increased the sensibility of traditional ELISA assay, reinforcing the idea that the use these nanomaterials are an excellent alternative for the immunoassays field.

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牛血清白蛋白在超顺磁/聚甲基丙烯酸甲酯纳米颗粒中的偶联作为磁性酶联免疫吸附测定的替代方法。

纳米材料，如磁性纳米颗粒，由于其提高免疫分析性能的能力而引起了医学界的极大关注。因此，本研究的目的是研究牛血清白蛋白(BSA)在超顺磁(MNPs)/聚甲基丙烯酸甲酯(PMMA)纳米颗粒上的偶联，并进一步表征其在酶联免疫吸附(ELISA)检测中的应用。通过光散射、zeta电位、透射电子显微镜(TEM)和Lowry蛋白定量分析等技术证实了BSA在MNPs- PMMA纳米颗粒上的成功偶联。用振动样品磁强计对其超顺磁性进行了验证。BSA偶联MNPs-PMMA纳米颗粒与抗体的相互作用高于游离BSA。BSA + MNPs-PMMA纳米颗粒(磁性酶联免疫吸附试验)减少了传统酶联免疫吸附试验的时间，提高了灵敏度，强化了使用这些纳米材料是免疫测定领域的一个极好的替代方案的想法。

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来源期刊

Journal of nanoscience and nanotechnology 工程技术-材料科学：综合

自引率

0.00%

发文量

审稿时长

3.6 months

期刊介绍： JNN is a multidisciplinary peer-reviewed journal covering fundamental and applied research in all disciplines of science, engineering and medicine. JNN publishes all aspects of nanoscale science and technology dealing with materials synthesis, processing, nanofabrication, nanoprobes, spectroscopy, properties, biological systems, nanostructures, theory and computation, nanoelectronics, nano-optics, nano-mechanics, nanodevices, nanobiotechnology, nanomedicine, nanotoxicology.