Faecal proteome in clinically healthy dogs and cats: Findings in pooled faeces from 10 cats and 10 dogs.

IF 1.3 Q2 VETERINARY SCIENCES

Veterinary Record Open Pub Date : 2021-04-07 eCollection Date: 2021-12-01 DOI:10.1002/vro2.9

Matteo Cerquetella, Andrea Marchegiani, Sara Mangiaterra, Giacomo Rossi, Alessandra Gavazza, Beniamino Tesei, Andrea Spaterna, Gianni Sagratini, Massimo Ricciutelli, Valeria Polzonetti, Stefania Pucciarelli, Silvia Vincenzetti

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Abstract

Background: In the scientific literature, there are only a few manuscripts available on small animal faecal proteomics.

Methods: In the present pilot study, this evaluation was performed using pooled faecal samples from 10 clinically healthy dogs and, for the first time, in 10 clinically healthy cats by mean of two-dimensional electrophoresis followed by liquid chromatography-tandem mass spectrometry.

Results: Our results showed the presence of nine (albumin, alkaline phosphatase, chymotrypsin-C-like, cytosol aminopeptidase, elastase-3B/proteinase E, immunoglobulins and nuclear pore membrane glycoprotein 210) and 14 (albumin, caspase recruitment domain-containing protein, chymotrypsin-like, deleted in malignant brain tumours 1 protein-like, hypothetical protein LOC107375, immunoglobulin, kallikrein-1, superoxide dismutase, transthyretin precursor, interstitial collagenase-like) different proteins in canine and feline faeces, respectively.

Conclusion: These preliminary findings document the presence of a range of proteins in the faeces of apparently healthy dogs and cats and may serve as a basis for larger, prospective studies to establish reference proteomic data against which diseased populations can be compared.

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临床健康猫狗的粪便蛋白质组：对 10 只猫和 10 只狗粪便的研究结果。

背景：在科学文献中，关于小动物粪便蛋白质组学的手稿寥寥无几：在本试验性研究中，通过二维电泳和液相色谱-串联质谱法，对 10 只临床健康犬和 10 只临床健康猫的粪便样本进行了评估：结果：我们的研究结果表明，猫体内存在 9 种（白蛋白、碱性磷酸酶、糜蛋白酶-C 样、细胞膜氨肽酶、弹性蛋白酶-3B/蛋白酶 E、免疫球蛋白和核孔膜糖蛋白 210）和 14 种（白蛋白、含 Caspase 招募结构域的蛋白、糜蛋白酶样、免疫球蛋白和核孔膜糖蛋白 210）蛋白质、犬和猫粪便中分别含有 14 种不同的蛋白质（白蛋白、含 Caspase 招募结构域的蛋白质、糜蛋白酶样蛋白、恶性脑肿瘤中删除的 1 类蛋白、假设蛋白 LOC107375、免疫球蛋白、卡利克雷因-1、超氧化物歧化酶、转甲状腺素前体、间质胶原酶样蛋白）。结论这些初步研究结果记录了表面上健康的狗和猫粪便中存在的一系列蛋白质，可作为更大规模的前瞻性研究的基础，以建立参考蛋白质组数据，并与患病人群进行比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Record Open VETERINARY SCIENCES-

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

19 weeks

期刊介绍： Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.