Hypnotic Effect of A. absinthium Hydroalcoholic Extract in Pentobarbital-Treated Mice.

IF 1.7 Q4 NEUROSCIENCES

Neurology Research International Pub Date : 2021-04-21 eCollection Date: 2021-01-01 DOI:10.1155/2021/5521019

Hassan Rakhshandeh, Amirhossein Heidari, Ali Mohammad Pourbagher-Shahri, Roghayeh Rashidi, Fatemeh Forouzanfar

{"title":"Hypnotic Effect of A. absinthium Hydroalcoholic Extract in Pentobarbital-Treated Mice.","authors":"Hassan Rakhshandeh, Amirhossein Heidari, Ali Mohammad Pourbagher-Shahri, Roghayeh Rashidi, Fatemeh Forouzanfar","doi":"10.1155/2021/5521019","DOIUrl":null,"url":null,"abstract":"Background: Current drugs used in the management of insomnia are associated with side effects. The use of medicinal herbs for insomnia treatment has recently been suggested.Objective: The present study aimed to determine the hypnotic activity of the hydroalcoholic extract of Artemisia absinthium (A. absinthium) in mice.Method: The toxicity of A. absinthium extract is assessed by their lethal dose 50% (LD50), and cytotoxicity evaluation was also done with PC12 cell lines by MTT assay. A. absinthium extract (25, 50, 100, and 200 mg/kg) and 3 fractions (n-butanol fraction (NBF), ethyl acetate fraction (EAF), and aqueous fraction (AQF)) were administered intraperitoneally30 minutes before 30 mg/kg pentobarbital intraperitoneal injection; after that, the sleeping time and sleep latency were recorded.Results: The LD50 value was 2.4 g/kg. The extracts tested showed no negative effect on the proliferation of PC12 cells. A. absinthium extract increased the duration of pentobarbital-induced sleep at doses of 100 and 200 mg/kg (P < 0.01-P < 0.001). Similarly, AQF, EAF, and NBF at 200 mg/kg could increase sleep duration (P < 0.05). The sleep latency was decreased by A. absinthium extract at doses of 100 and 200 mg/kg (P < 0.05-P < 0.01), AQF (P < 0.05), and EAF (P < 0.05). Besides, flumazenil reversed the hypnotic effect of A. absinthium extract (P < 0.05).Conclusion: A. absinthium extract probably demonstrated sleep-enhancing effects by regulating GABAergic system.","PeriodicalId":19124,"journal":{"name":"Neurology Research International","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2021-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084640/pdf/","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurology Research International","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2021/5521019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}

引用次数: 6

Abstract

Background: Current drugs used in the management of insomnia are associated with side effects. The use of medicinal herbs for insomnia treatment has recently been suggested.

Objective: The present study aimed to determine the hypnotic activity of the hydroalcoholic extract of Artemisia absinthium (A. absinthium) in mice.

Method: The toxicity of A. absinthium extract is assessed by their lethal dose 50% (LD50), and cytotoxicity evaluation was also done with PC12 cell lines by MTT assay. A. absinthium extract (25, 50, 100, and 200 mg/kg) and 3 fractions (n-butanol fraction (NBF), ethyl acetate fraction (EAF), and aqueous fraction (AQF)) were administered intraperitoneally30 minutes before 30 mg/kg pentobarbital intraperitoneal injection; after that, the sleeping time and sleep latency were recorded.

Results: The LD50 value was 2.4 g/kg. The extracts tested showed no negative effect on the proliferation of PC12 cells. A. absinthium extract increased the duration of pentobarbital-induced sleep at doses of 100 and 200 mg/kg (P < 0.01-P < 0.001). Similarly, AQF, EAF, and NBF at 200 mg/kg could increase sleep duration (P < 0.05). The sleep latency was decreased by A. absinthium extract at doses of 100 and 200 mg/kg (P < 0.05-P < 0.01), AQF (P < 0.05), and EAF (P < 0.05). Besides, flumazenil reversed the hypnotic effect of A. absinthium extract (P < 0.05).

Conclusion: A. absinthium extract probably demonstrated sleep-enhancing effects by regulating GABAergic system.

查看原文本刊更多论文

苦艾水醇提取物对戊巴比妥治疗小鼠的催眠作用。

背景:目前用于治疗失眠的药物存在副作用。最近有人建议用草药治疗失眠。目的:研究苦艾水醇提取物对小鼠的催眠作用。方法:以50%致死剂量(LD50)评价苦艾提取物的毒性，并采用MTT法对PC12细胞株进行细胞毒性评价。A.苦艾草提取物(25、50、100、200 mg/kg)和3个部分(正丁醇部分(NBF)、乙酸乙酯部分(EAF)、水溶液部分(AQF))在戊巴比妥30 mg/kg腹腔注射前30分钟腹腔注射;之后，记录睡眠时间和睡眠潜伏期。结果:LD50值为2.4 g/kg。所测提取物对PC12细胞的增殖无负面影响。苦艾草提取物在100和200 mg/kg剂量下增加戊巴比妥诱导的睡眠持续时间(P < 0.01-P < 0.001)。同样，200 mg/kg的AQF、EAF和NBF也能增加睡眠时间(P < 0.05)。苦艾草提取物100、200 mg/kg组(P < 0.05 ~ P < 0.01)、AQF (P < 0.05)和EAF (P < 0.05)均能显著降低大鼠睡眠潜伏期。苦艾草提取物(P < 0.05)。结论:苦艾草提取物可能通过调节gaba能系统发挥促睡眠作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Neurology Research International NEUROSCIENCES-

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

17 weeks

期刊介绍： Neurology Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies focusing on diseases of the nervous system, as well as normal neurological functioning. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.