Granulocyte-colony stimulating factor, a potential candidate for the treatment of Parkinson's disease.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-01 Epub Date: 2021-05-03 DOI:10.23736/S0390-5616.21.05314-5

Jiawen Yuan, Li-Xia Xue, Jin-Peng Ren

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引用次数: 0

Abstract

Background: Granulocyte colony-stimulating factor (G-CSF) activates the PI3K/Akt pathway to exert neuroprotective effects. The current study aimed to determine if G-CSF reverses behavioral deficits, even after motor malfunction occurs in Paraquat (PQ)-treated mice.

Methods: Male C57BL/6 mice (8 weeks old) were divided into 3 groups: PQ + G-CSF-treated group (N.=8); PQ + saline-treated group (N.=8); and saline-treated control group (N.=8). Spontaneous locomotor activity was evaluated together with the pole test. The DA, 3, 4-dihydroxyphenyl acetic acid (DOPAC), and homovanillic acid (HVA) levels in the bilateral striatum were determined by HPLC. The number of substantia nigra pars compacta tyrosine hydroxylase (TH)-immunoreactive neurons was calculated using an unbiased cell counting stereology method, the activities of total GSH-PX and SOD, and the malondialdehyde (MDA) content were assessed.

Results: After G-CSF treatment, spontaneous motor activity and the Tturn and TLA times in the CSF group were significantly lower than the control group, and the striatal dopamine level in the striatum and the number of TH-positive neurons in the substantia nigra (SN) were significantly increased compared to the control group (5478±654 vs. 3647±488 DA neurons, P<0.05). Compared to the control group, the GSH-PX and SOD activities were increased, while the MDA level was significantly decreased in the SN (P<0.05).

Conclusions: The data strongly suggest that G-CSF reverses behavioral deficits in PQ-treated mice with movement disorders. Thus, G-CSF may be utilized as a prospective drug candidate for the treatment of Parkinson's disease.

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治疗帕金森病的潜在候选药物--粒细胞集落刺激因子。

背景：粒细胞集落刺激因子（G-CSF粒细胞集落刺激因子（G-CSF）可激活 PI3K/Akt 通路，从而发挥神经保护作用。本研究旨在确定即使在百草枯（PQ）处理的小鼠出现运动功能障碍后，G-CSF是否仍能逆转其行为障碍：雄性 C57BL/6 小鼠（8 周大）分为 3 组：方法：雄性C57BL/6小鼠（8周龄）分为3组：百草枯+G-CSF处理组（n=8）；百草枯+生理盐水处理组（n=8）；生理盐水处理对照组（n=8）。自发运动活动与极点试验一起评估。双侧纹状体中的DA、3, 4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）水平由高效液相色谱法测定。采用无偏差细胞计数立体法计算黑质酪氨酸羟化酶（TH）免疫反应神经元的数量，评估总 GSH-PX 和 SOD 的活性以及丙二醛（MDA）的含量：G-CSF治疗后，CSF组的自发运动活动、Tturn和TLA时间明显低于对照组，纹状体中多巴胺水平和黑质（SN）中TH阳性神经元数量明显高于对照组（5478±654 vs. 3647±488 DA神经元，P＜0.05）。与对照组相比，SN 中 GSH-PX 和 SOD 活性增加，而 MDA 水平明显下降（PConclusions：这些数据有力地表明，G-CSF 可逆转 PQ 治疗的运动障碍小鼠的行为缺陷。因此，G-CSF 可作为治疗帕金森病的候选药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.