{"title":"A method of restoration and preservation of mounted anatomical pathology specimens.","authors":"Manisha Kulkarni, Pradeep Vaideeswar","doi":"10.1080/01478885.2021.1884352","DOIUrl":null,"url":null,"abstract":"<p><p>Big hospitals and biology museums often have a sizable collection of valuable archived anatomical pathology specimens, collected over a long period of time. Traditionally, these specimens are suspended by thread tied to a frame of glass rods in a rectangular glass jar. While restoring with preserving solution, specimens often fell from sewn thread or strings. We developed an easy method of remounting such specimens on Perspex sheets with cyanoacrylate adhesive. This technique was tested on five archived specimens and five recent well-fixed specimens, including brain. Specimens could be easily oriented, remounted and remained well adhered to the Perspex sheet. Gross pathologic characteristics could be well illustrated from all sides in the remounted specimen. Cyanoacrylate adhesive technique was useful in permanent preservation of old delicate anatomical pathology specimens, which could not be tied to glass rods again and had fallen out of position in mounting jars. This technique can also be applied for well-fixed recent specimens. It is easy, cost effective, serves as an educational tool and can be applied to all types of specimens.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"144-149"},"PeriodicalIF":0.6000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1884352","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Histotechnology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/01478885.2021.1884352","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/5/17 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Big hospitals and biology museums often have a sizable collection of valuable archived anatomical pathology specimens, collected over a long period of time. Traditionally, these specimens are suspended by thread tied to a frame of glass rods in a rectangular glass jar. While restoring with preserving solution, specimens often fell from sewn thread or strings. We developed an easy method of remounting such specimens on Perspex sheets with cyanoacrylate adhesive. This technique was tested on five archived specimens and five recent well-fixed specimens, including brain. Specimens could be easily oriented, remounted and remained well adhered to the Perspex sheet. Gross pathologic characteristics could be well illustrated from all sides in the remounted specimen. Cyanoacrylate adhesive technique was useful in permanent preservation of old delicate anatomical pathology specimens, which could not be tied to glass rods again and had fallen out of position in mounting jars. This technique can also be applied for well-fixed recent specimens. It is easy, cost effective, serves as an educational tool and can be applied to all types of specimens.
期刊介绍:
The official journal of the National Society for Histotechnology, Journal of Histotechnology, aims to advance the understanding of complex biological systems and improve patient care by applying histotechniques to diagnose, prevent and treat diseases.
Journal of Histotechnology is concerned with educating practitioners and researchers from diverse disciplines about the methods used to prepare tissues and cell types, from all species, for microscopic examination. This is especially relevant to Histotechnicians.
Journal of Histotechnology welcomes research addressing new, improved, or traditional techniques for tissue and cell preparation. This includes review articles, original articles, technical notes, case studies, advances in technology, and letters to editors.
Topics may include, but are not limited to, discussion of clinical, veterinary, and research histopathology.